Doglia / Lotti Protein Aggregation in Bacteria

Functional and Structural Properties of Inclusion Bodies in Bacterial Cells
1. Auflage 2014
ISBN: 978-1-118-85503-4
Verlag: John Wiley & Sons
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 284 Seiten, E-Book

Reihe: Wiley Series in Protein and Peptide Science

Focuses on the aggregation of recombinant proteins inbacterial cells in the form of inclusion bodies--and on theiruse in biotechnological and medical applications
The first book devoted specifically to the topic of aggregationin bacteria, Protein Aggregation in Bacteria: Functional andStructural Properties of Inclusion Bodies in Bacterial Cellsprovides a large overview of protein folding and aggregation,including cell biology and methodological aspects. It summarizes,for the first time in one book, ideas and technical approaches thatpave the way for a direct use of inclusion bodies inbiotechnological and medical applications.
Protein Aggregation in Bacteria covers:
* Molecular and cellular mechanisms of protein folding,aggregation, and disaggregation in bacteria
* Physiological importance and consequences of aggregation forthe bacterial cell
* Factors inherent to the protein sequence responsible foraggregation and evolutionary mechanisms to keep proteinssoluble
* Structural properties of proteins expressed as solubleaggregates and as inclusion bodies within bacterial cells both froma methodological point of view and with regard to their similaritywith amyloids
* Control of the structural and functional properties ofaggregated proteins and use thereof in biotechnology andmedicine
Protein Aggregation in Bacteria is ideal for researchersin protein science, biochemistry, bioengineering, biophysics,microbiology, medicine, and biotechnology, particularly if they arerelated with the production of recombinant proteins andpharmaceutical science.

Autoren/Hrsg.

Doglia, Silvia Maria

Lotti, Marina

Inhaltsverzeichnis

Contributors vii
Preface xi
Introduction to the Wiley Series in Protein and Peptide Sciencexiii
1 Fundamentals of Protein Folding 1
Vladimir N. Uversky
2 Recruiting Unfolding Chaperones to Solubilize MisfoldedRecombinant Proteins 63
Rayees U.H. Mattoo and Pierre Goloubinoff
3 Osmolytes as Chemical Chaperones to Use in ProteinBiotechnology 77
Ario de Marco
4 Inclusion Bodies in the Study of Amyloid Aggregation 93
Anna Villar-Piqué and Salvador Ventura
5 Protein Aggregation in Unicellular Eukaryotes 117
Marina Caldara, Joris Winderickx, and Vanessa Franssens
6 Structural Properties of Bacterial Inclusion Bodies 151
Antonino Natalello, Diletta Ami, and Silvia Maria Doglia
7 Residue-Specific Structural Studies of Inclusion Bodies181
Christian Wasmer, Marielle Wälti, Yongli Chen, and LeiWang
8 Biomedical Applications of Bacterial Inclusion Bodies203
Imma Ratera, Spela Peternel, Joaquin Seras-Franzoso, OliviaCano-Garrido, Elena García-Fruitós, Rafael Cubarsí,Esther Vazquez, José Luis Corchero, EscarlataRodríguez-Carmona, Jaume Veciana, and Antonio Villaverde
9 Aggregation of Recombinant Proteins: Understanding BasicIssues to Overcome Production Bottlenecks 221
Marina Lotti and Loredano Pollegioni
10 Fusion to a Pull-Down Module: Designing Enzymes to FormBiocatalytically Active Insoluble Aggregates 247
Bernd Nidetzky
Index 263

Über Autor(innen)

SILVIA MARIA DOGLIA, PhD, is Professor of Physics at theUniversity of Milano-Bicocca, Italy. She received her Laurea degreein Physics at the University of Milano. She has been Staff ResearchScientist of the Italian National Research Council; VisitingScientist at the University of Stockholm; Visiting Professor at theUniversities of Orléans (Fr) and of Reims (Fr). Her researchin Biophysics at the University of Milano-Bicocca is focused on thestudy of protein folding and aggregation in vitro and insitu.
MARINA LOTTI, PhD, is Professor of Biochemistry at theUniversity of Milano-Bicocca, Italy, where she leads the groupProtein Engineering and Industrial Enzymology, and is the Head ofthe Department of Biotechnology and Biosciences. She obtained herPhD degree at the Max-Planck Institute of Molecular Genetics,Berlin, and was a researcher of the Italian National ResearchCouncil. Major research topics include the production ofrecombinant proteins, protein aggregation, cold-active enzymes, andintrinsically disordered proteins.