Déjardin Proteins at Solid-Liquid Interfaces

1. Auflage 2006
ISBN: 978-3-540-32658-8
Verlag: Springer Berlin Heidelberg
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 330 Seiten

Reihe: Principles and Practice

This book opens with a description of fundamental aspects of protein adsorption to surfaces, a phenomenon that plays a key role in biotechnological applications, especially at solid-liquid interfaces. Presented here are methods for studying adsorption kinetics and conformational changes such as optical waveguide lightmode spectroscopy (OWLS). Also described are sensitive bench techniques for measuring the orientation and structure of proteins at solid-liquid interfaces, including total internal reflection ellipsometry (TIRE), dual polarisation interferometry (DPI) and time of flight - secondary ion mass spectrometry (TOF–SIMS). A model study of fibronectin at polymer surfaces is included, as are studies using microporous membranes and textiles with immobilized enzymes for large-scale applications. Biocompatibility, anti-fouling properties and surface modification to modulate the adsorption and activity of biomolecules are among the other topics addressed in this invaluable book.

Autoren/Hrsg.

Déjardin, Philippe

Inhaltsverzeichnis

1;Preface;5
2;Contents;7
3;Contributors;13
4;Protein Adsorption Kinetics: Influence of Substrate Electric Potential;16
4.1;1.1 Introduction;16
4.2;1.2 Theoretical Prediction;17
4.3;1.3 Experimental Measure;21
4.4;1.4 Results;24
4.5;1.5 Discussion;32
4.6;1.6 Conclusions;36
4.7;References;36
5;From Kinetics to Structure: High Resolution Molecular Microscopy;38
5.1;2.1 Introduction;38
5.2;2.2 OpticalWaveguide Lightmode Spectroscopy;40
5.3;2.3 The Practical Determination ofWaveguide Parameters;49
5.4;2.4 Static Structure;52
5.5;2.5 Kinetic Analysis and Dynamic Structural Inference;52
5.6;2.6 Behaviour of Real Proteins;58
5.7;2.7 Conclusions;62
5.8;References;63
6;Initial Adsorption Kinetics in a Rectangular Thin Channel, and Coverage- Dependent Structural Transition Observed by Streaming Potential;66
6.1;3.1 Introduction;66
6.2;3.2 The Initial Adsorption Constant and its Limit Expressions;71
6.3;3.3 The Structural Transition with Increasing Interfacial Concentration;78
6.4;3.4 Conclusion;82
6.5;Appendix;83
6.6;References;84
7;Dual Polarisation Interferometry: An Optical Technique to Measure the Orientation and Structure of Proteins at the Solid– Liquid Interface in Real Time;90
7.1;4.1 Introduction;90
7.2;4.2 Experimental Approaches Adopted;94
7.3;4.3 DPI: Applications;95
7.4;4.4 Future Developments;106
7.5;4.5 Conclusions;108
7.6;Appendix 1 DPI: Background;108
7.7;Appendix 2 DPI: Theory;110
7.8;Appendix 3 DPI: Implementation;114
7.9;References;117
8;Total Internal Reflection Ellipsometry: Monitoring of Proteins on Thin Metal Films;120
8.1;5.1 Introduction;120
8.2;5.2 Total Internal Reflection Ellipsometry;121
8.3;5.3 Experimental Setup;125
8.4;5.4 Application Examples;128
8.5;5.5 Further Possibilities;132
8.6;References;133
9;Conformations of Proteins Adsorbed at Liquid– Solid Interfaces;134
9.1;6.1 Introduction;134
9.2;6.2 Experimental Techniques;140
9.3;6.3 Surface Effects on Both Protein Structure and Solvation by the ATR- FTIR Technique;145
9.4;6.4 Conclusion;157
9.5;References;157
10;Evaluation of Proteins on Bio-Devices;166
10.1;7.1 Introduction;166
10.2;7.2 Time-of-Flight Secondary Ion Mass Spectrometry ( TOF- SIMS);168
10.3;7.3 Analysis of Proteins on Bio-Devices;176
10.4;7.4 Summary;184
10.5;References;184
11;Fibronectin at Polymer Surfaces with Graduated Characteristics;190
11.1;8.1 Introduction;190
11.2;8.2 Gradated Substrate Physicochemistry;192
11.3;8.3 Fibronectin Exchange at a Constant Surface Concentration;196
11.4;8.4 Fibronectin Exchange at Variable Surface Concentrations;203
11.5;8.5 Relevance of the Interfacial Constraints of Fibronectin for Cell- Matrix Adhesion;210
11.6;References;212
12;Development of Chemical Microreactors by Enzyme Immobilization onto Textiles;214
12.1;9.1 Introduction;214
12.2;9.2 Nonconducting Cellulosic Textiles;216
12.3;9.3 Electron-Conducting Textile;242
12.4;References;257
13;Approaches to Protein Resistance on the Polyacrylonitrile- based Membrane Surface: an Overview;260
13.1;10.1 Introduction;260
13.2;10.2 Copolymerization Procedures;261
13.3;10.3 Poly(ethylene glycol) Tethering;267
13.4;10.4 Physical Adsorption;272
13.5;10.5 Biomacromolecule Immobilization;274
13.6;10.6 Biomimetic Modification;278
13.7;10.7 Conclusion;281
13.8;References;283
14;Modulation of the Adsorption and Activity of Protein/ Enzyme on the Polypropylene Microporous Membrane Surface by Surface Modification;286
14.1;11.1 Surface Modifications for Reducing Nonspecific Protein Adsorption;286
14.2;11.2 Surface-Modified PPMMs for Enzyme Immobilization;301
14.3;11.3 Conclusions;310
14.4;References;310
15;Nonbiofouling Surfaces Generated from Phosphorylcholine- Bearing Polymers;314
15.1;12.1 Introduction;314
15.2;12.2 Forces Involved in Protein Adsorption;315
15.3;12.3 Design of Phosphorylcholine-Bearing Surfaces;317
15.4;12.4 Mechanism of Resistance to Protein Adsorption on the MPC Polymer Surface;318
15.5;12.5 Fundamental Interactions Between MPC Polymers and Proteins;325
15.6;12.6 Recent Designs of Nonfouling Phosphorylcholine Surfaces withWell- Defined Structures;327
15.7;12.7 Control of Cell–Material Interactions on a Phosphorylcholine Polymer Nonfouling Surface;329
15.8;12.8 Conclusion;336
15.9;References;336
16;Subject Index;342