Desai Downstream Processing of Proteins

Methods and Protocols
2000
ISBN: 978-1-59259-027-8
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 9, 229 Seiten, eBook

Reihe: Methods in Biotechnology

Considerable effort and time is allocated to introducing cell culture and fermentation technology to undergraduate students in academia, generally through a range of courses in industrial biotechnology and related disciplines. Similarly, a large number of textbooks are available to describe the appli- tions of these technologies in industry. However, there has been a general lack of appreciation of the significant developments in downstream processing and isolation technology, the need for which is largely driven by the stringent re- latory requirements for purity and quality of injectable biopharmaceuticals. This is particularly reflected by the general absence of coverage of this s- ject in many biotechnology and related courses in educational institutions. For a considerable while I have felt that there is increasing need for an introductory text to various aspects of downstream processing, particularly with respect to the needs of the biopharmaceutical and biotechnology ind- try. Although there are numerous texts that cover various aspects of protein purification techniques in isolation, there is a need for a work that covers the broad range of isolation technology in an industrial setting. It is anticipated that Downstream Processing of Proteins: Methods and Protocols will play a small part in filling this gap and thus prove a useful contribution to the field. It is also designed to encourage educational strategists to broaden the coverage of these topics in industrial biotechnology courses by including accounts of this important and rapidly developing element of the industrial process.

Zielgruppe

Research

Autoren/Hrsg.

Desai, Mohamed A.

Inhaltsverzeichnis

Downstream Processing in the Biotechnology Industry: An Overview, Manohar Kalyanpur. Microbial Cell Disruption by High-Pressure Homogenization, Anton P. J. Middelberg. Protein Concentration and Buffer Exchange Using Ultrafiltration, Andrew L. Zydney and Ralf Kuriyel. High-Performance Tangential Flow Filtration for Protein Separations, Andrew L. Zydney and Ralf Kuriyel. Large-Scale Recovery of Protein Inclusion Bodies by Continuous Centrifugation, Anton P. J. Middelberg. Application of Density Gradient Ultracentrifugation Using Zonal Rotors in the Large-Scale Purification of Biomolecules, Mohamed A. Desai and Sandra P. Merino. Application of Chromatography in the Downstream Processing of Biomolecules, Mohamed A. Desai, Mark Rayner, Mike Burns, and Dervilla Bermingham. Affinity Ligand Technology: Optimal Protein Separations for Downstream Processing, Ken Jones and Dev Baines. Immunoaffinity Adsorption in the Large-Scale Isolation of Biomolecules, Mohamed A. Desai. Expanded Bed Adsorption in the Purification of Biomolecules, Allan Lihme, Marie Hansen, Morten Olander, and Elias Zafirakos. Application of HPLC in the Purification of Biomolecules, Paul Bradley and Mohamed A. Desai. Countercurrent Chromatography of Proteins with Polymer Phase Systems, Yoichiro Shibusawa, Kazuhiro Matsuda, and Yoichiro Ito. Practical Aspects and Applications of Radial Flow Chromatography, Denise M. Wallworth. Sterile Filtration and Virus Filtration, Ralf Kuriyel and Andrew L. Zydney. Design and Interpretation of Viral Clearance Studies for Biopharmaceutical Products, Allan J. Darling. Validation of Sterilizing Filters in the Biotechnology Industry, Manohar Kalyanpur. Index.