Electrical Manipulation of Cells

1996
ISBN: 978-0-412-03001-7
Verlag: Springer US

Buch, Englisch, 292 Seiten, HC runder Rücken kaschiert, Format (B × H): 160 mm x 241 mm, Gewicht: 1350 g

provides an authoritative and up-to-date review of the field, covering all the major techniques in a single source. The book features broad coverage that ranges from the mechanisms of action of external electrical fields on biological material to the ways in which electrical stimuli are employed to manipulate cells. Bringing together the work of leading international authorities, the book covers membrane breakdown, gene delivery, electroporation, electrostimulation, cell movement, hybridoma production, plant protoplasts, electrorotation and stimulation, and electromagnetic stimulation.
For each topic, the authors discuss the relevance of the approach to the current state of the art of biotechnology. is an unmatched source of information for anyone involved in the manipulation of cells, particularly biotechnologists, cell biology, microbiologists, biophysicists and plant scientists. For researchers, the book provides technical material that ccan be employed in their own work. Students will gain thorough appreciation of the applications of this important technique.

Zielgruppe

Research

Autoren/Hrsg.

Davey, M. R.

Lynch, Paul T.

Fachgebiete

• Naturwissenschaften Biowissenschaften Proteinforschung
• Naturwissenschaften Biowissenschaften Botanik Pflanzenphysiologie, Photosynthese
• Naturwissenschaften Biowissenschaften Tierkunde / Zoologie Tierethologie
• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

1 Electrofusion and Electroporation Equipment.- 2 Electrically Stimulated Membrane Breakdown.- 3 Cell Electrorotation.- 4 Dielectrophoresis: Effect of Nonuniform Electrical Fields on Cell Movement.- 5 Electrofusion of Animal Cells.- 6 Cell-Tissue Electrofusion.- 7 Electrofusion of Plant Protoplasts for Hybrid Plant Production.- 8 Gene Delivery by Membrane Electroporation.- 9 Clinical Applications of Electroporation.- 10 Electroporation and Transgenic Plant Production.- 11 Electromagnetic Cell Stimulation.- 12 Electrostimulation of Cells by Weak Electric Currents.- 13 Stimulation of Plant Cell Division and Organogenesis by Short-Term, High-Voltage Electrical Pulses.