Daadi Neural Stem Cells

Methods and Protocols
Erscheinungsjahr 2019
ISBN: 978-1-4939-9007-8
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1919, 239 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume details various technological advances to isolate, perpetuate, and characterize neural stem cells from various sources and formulates them for the delivery into animal models of neurological disorders. Chapters guide the reader through methods and protocols on definition and isolation of neural stem cells, characterization and differentiation, standards for defining cell populations, and In vivo approaches in animal models.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Neural Stem Cells: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Daadi, Marcel M.

Inhaltsverzeichnis

Generation of Neural Stem Cells from Induced Pluripotent Stem Cells.- Making NSC and Neurons from Patient Derived Tissue Samples.- Generation of definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury.- Derivation of Neural Stem Cells from Human Parthenogenetic Stem Cells.- Chemically Defined Neural Conversion of Human Pluripotent Stem Cells.- In vitro Functional Characterization of Human Neurons and Astrocytes Using Calcium Imaging and Electrophysiology.- Differentiation of Neural Stem Cells Derived from Induced Pluripotent Stem Cells into Dopaminergic Neurons.- Reference Transcriptome for Deriving Nonhuman Primate Induced Pluripotent Stem Cells.- Generating Neural Stem Cells from iPSCs with Dopaminergic Neurons Reporter Gene.- Single-Cell Library Preparation of iPSC-derived Neural Stem Cells.- Bioinformatic Analysis of Single-Cell RNA-Seq Raw Data From iPSC-Derived Neural Stem Cells.- Assay for Assessing Mitochondrial Function In iPSC-Derived Neural Stem Cells and Dopaminergic Neurons.- Standards for Deriving Nonhuman Primate Induced Pluripotent Stem Cells.- Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach.- Isolation and Differentiation of Self-Renewable Neural Stem Cells from Marmoset Induced Pluripotent Stem Cells.- Lentiviral Infection of Mouse Bone Marrow Cells for Hematopoietic Stem Cell Transplantation.- Central and Peripheral Secondary Cell Death Processes After Transient Global Ischemia in Non-human Primate Cerebellum and Heart.- Method for Stimulation of Hippocampal Neurogenesis by Transient Micro-needle Insertion.