Crawley What's Wrong With My Mouse?

Behavioral Phenotyping of Transgenic and Knockout Mice
2. Auflage 2007
ISBN: 978-0-470-11904-4
Verlag: John Wiley & Sons
Format: PDF
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 544 Seiten, E-Book

Dr. Jacqueline N. Crawley, author of the First and Second Editionsof What's Wrong with My Mouse? Behavioral Phenotyping ofTransgenic and Knockout Mice,continues to field calls ande-mails from molecular geneticists who ask: how do I runbehavioral assays to find out what's wrong with my mouse?
Turn to What's Wrong with My Mouse? to discover thewealth of mouse behavioral tasks and to get the guidance you needto select the best methods and necessary controls. Chapters areorganized by behavioral domain, including measurements of generalhealth, motor functions, sensory abilities, learning and memory,feeding and drinking, reproductive, social, emotional, and rewardbehaviors in mutant mice. Throughout the chapters, new behavioraltasks and new research discoveries have been added, bringing theSecond Edition up to date with the latest science. In addition, theSecond Edition includes two new chapters:
"Neurodevelopment and Neurodegeneration" discusses mousebehavioral tasks relevant to neurodevelopmental diseases, such asmental retardation and autism, and to neurodegenerative diseases,such as Alzheimers, Parkinsons, Huntingtons, and amyotrophiclateral sclerosis.
"Putting It All Together" recommends strategies for optimizing abattery of behavioral phenotyping tests to address your specifichypotheses about gene functions.
The final chapter, "The Next Generation," examines new andemerging technologies.
Throughout the book, the use of behavioral testing equipment isillustrated with photographs, diagrams, and representative data.Examples of behavioral tasks successfully applied to transgenic andknockout mouse models are provided, as well as references to theprimary literature and step-by-step methods protocols. Thesefeatures, along with a comprehensive index, listings of databaseand vendor websites, and an extensive list of references, make thisbook a valuable and practical resource for students andresearchers.

Autoren/Hrsg.

Crawley, Jacqueline N.

Inhaltsverzeichnis

Preface ix
Preface to the First Edition xi
Acknowledgments xiii
Chapter 1 Designer Mice 1
Scope and sourcebooks
Chapter 2 Of Unicorns and Chimeras 10
How to generate a line of transgenic or knockout mouse for behavioral phenotyping
Chapter 3 General Health 42
Give your mouse a physical
Chapter 4 Motor Functions 62
Open field, holeboard, rotarod, balance, grip, circadian activity, circling, stereotypy, ataxic gait, seizures
Chapter 5 Sensory Abilities 86
Olfaction, vision, hearing, taste, touch, nociception
Chapter 6 Learning and Memory 110
Morris swim task, spatial mazes, cued and contextual conditioning, conditioned taste aversion, conditioned eyeblink, olfactory discrimination, social recognition, passive avoidance, schedule controlled operant tasks, motor learning, attention
Chapter 7 Feeding and Drinking 164
Daily consumption, restricted access, choice tests, microstructural analysis
Chapter 8 Reproductive Behaviors 186
Sexual and parental behaviors
Chapter 9 Social Behaviors 206
Social interaction, nesting, grooming, juvenile play, aggression
Chapter 10 Emotional Behaviors: Animal Models of Psychiatric Diseases 226
Mouse models of fear, anxiety, depression, schizophrenia
Chapter 11 Reward 266
Self-administration of addictive drugs, conditioned place preference
Chapter 12 Neurodevelopment and Neurodegeneration 290
Assaying behaviors in infant, juvenile, and aged mice
Chapter 13 Putting It All Together 322
Choice of tests, order of testing, number of mice, equipment, housing and testing environment
Chapter 14 The Next Generation 344
Conditional and inducible mutations, viral vector gene delivery, RNA silencing, quantitative trait loci analysis, DNA microarrays, chemical mutagenesis, gene therapy, ethical issues
References 383
Index 503

Über Autor(innen)

Jacqueline N. Crawley, PhD, is Chief of the Laboratory ofBehavioral Neuroscience at the National Institute of Mental Health;Adjunct Professor of Psychiatry and Psychology at the University ofNorth Carolina at Chapel Hill; and Adjunct Professor ofPharmacology at Georgetown University. Dr. Crawley has publishedmore than 200 peer-reviewed papers and 60 review articles. Herresearch has included rodent behavioral analyses of noradrenergicpathways; benzodiazepine receptor ligands; neuropeptidetransmitters, including galanin and cholecystokinin; mouse geneticmodels of autism; and behavioral phenotyping of more than 30 linesof transgenic and knockout mice. Recent awards include theInternational Behavioral Neuroscience Society Myers LifetimeAchievement Award (2005), the Fleur Strand Summer NeuropeptideConference Award (2005), the Howard Hughes Medical InstitutePreceptor Award (2004), and the National Institute of Mental HealthDirector's Award (2002).
What's Wrong With My Mouse?: Behavioral Phenotyping ofTransgenic and Knockout Mice, Second Edition has been written inDr. Crawley's private capacity, outside of her professionalposition at the National Institutes of Health. The views expressedin this book do not necessarily represent the views of the NationalInstitutes of Health or of the United States government.