Buch, Englisch, 234 Seiten, Format (B × H): 160 mm x 241 mm, Gewicht: 587 g
Reihe: Methods in Molecular Biology
Buch, Englisch, 234 Seiten, Format (B × H): 160 mm x 241 mm, Gewicht: 587 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-58829-533-0
Verlag: Humana Press
The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge and new techniques that can be adapted easily to different molecules and cell types. The topics discussed in this updated second edition include novel techniques for studying cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to study leukocyte migration through monolayers of cultured endothelial cells, and novel techniques to purify pseudopodia from migratory cells. This book also discusses the study of cell-matrix interaction, RNA interference, fluorescence recovery after photobleaching, actin purification, the application of microarray techniques, and the role of adhesion proteins in the study of proteomics. The protocols discussed in this volume are suitable for both novice and expert scientists, who will gain further insight into the complex and incompletely understood processes involved in cellular adhesion.
Zielgruppe
Research
Autoren/Hrsg.
Fachgebiete
- Naturwissenschaften Biowissenschaften Proteinforschung
- Naturwissenschaften Biowissenschaften Zellbiologie
- Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
- Naturwissenschaften Biowissenschaften Angewandte Biologie Bioinformatik
- Mathematik | Informatik EDV | Informatik Angewandte Informatik Bioinformatik
Weitere Infos & Material
Manipulation of Cell-Cell Adhesion Using Bowtie-Shaped Microwells.- Separation of Cell-Cell Adhesion Complexes by Differential Centrifugation.- Analysis of Neutrophil Chemotaxis.- Analysis of Leukocyte Migration Through Monolayers of Cultured Endothelial Cells.- Biochemical Purification of Pseudopodia from Migratory Cells.- Dynamic Assessment of Cell-Matrix Mechanical Interactions in Three-Dimensional Culture.- Quantitative Analyses of Cell Adhesion Strength.- Using RNA Interference to Knock Down the Adhesion Protein TES.- Assaying Calpain Activity.- Analysis of Focal Adhesions and Cytoskeleton by Custom Microarray.- Proteomic Analysis of Cell Surface Membrane Proteins in Leukemic Cells.- Bioinformatic Analysis of Adhesion Proteins.- Analysis of Integrin Dynamics by Fluorescence Recovery After Photobleaching.- Double-Hydrogel Substrate as a Model System for Three-Dimensional Cell Culture.- In Vitro Actin Assembly Assays and Purification From Acanthamoeba.
