Cooper Amino Acid Analysis Protocols

Erscheinungsjahr 2008
ISBN: 978-1-59259-047-6
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 159, 265 Seiten, eBook

Reihe: Methods in Molecular Biology

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Cooper, Catherine

Inhaltsverzeichnis

Amino Acid Analysis.- Amino Acid Analysis, Using Postcolumn Ninhydrin Detection, in a Biotechnology Laboratory.- Purification of Proteins Using UltraMacro Spin Columns or ProSorb Sample Preparation Cartridges for Amino Acid Analysis.- Amino Acid Analysis Using Precolumn Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate.- Amino Acid Analysis by High-Performance Liquid Chromatography after Derivatization with 1-Fluoro-2,4-Dinitrophenyl-5-L-Alanine Amide (Marfey's Reagent).- The Analysis of Amino Acids Using Precolumn Derivatization, HPLC, and Electrochemical Detection.- Anion Exchange Chromatography and Integrated Amperometric Detection of Amino Acids.- Ion-Pair Chromatography for Identification of Picomolar-Order Protein on a PVDF Membrane.- Capillary Gas Chromatographic Analysis of Protein and Nonprotein Amino Acids in Biological Samples.- Measurement of Blood Plasma Amino Acids in Ultrafiltrates by High-Performance Liquid Chromatography with Automatic Precolumn O-Phthaldialdehyde Derivatization.- Determination of Amino Acids in Foods by Reversed-Phase High-Performance Liquid Chromatography with New Precolumn Derivatives, Butylthiocarbamyl, and Benzylthiocarbamyl Derivatives Compared to the Phenylthiocarbamyl Derivative and Ion Exchange Chromatography.- Amino Acid Measurement in Body Fluids Using PITC Derivatives.- Determination of Proteins, Phosphatidylethanolamine, and Phosphatidylserine in Lipid-Rich Materials by Analysis of Phenylthiocarbamyl Derivatives.- Analysis of O-Phosphoamino Acids in Biological Samples by Gas Chromatography with Flame Photometric Detection.- Determination of Sulfur Amino Acids, Glutathione, and Related Aminothiols in Biological Samples by Gas Chromatography with Flame Photometric Detection.- Capillary Electrophoretic Determination of 4-Hydroxyproline.- Total Plasma Homocysteine Analysis by HPLC with SBD-F Precolumn Derivatization.- Determination of Early Glycation Products by Mass Spectrometry and Quantification of Glycation Mediated Protein Crosslinks by the Incorporation of [14C]lysine into Proteins.