Conn The Receptors

Volume II
1. Auflage 2014
ISBN: 978-1-4832-7368-6
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 454 Seiten, Web PDF

The Receptors, Volume II deals with receptors for somatostatin, vitamin D, insulin, and animal viruses, as well as for the ?2-adrenergic and Ah systems. The significance of translational modifications of receptor ligands is discussed, along with the mechanisms of receptor-ligand interactions. The role of receptors in development and their regulation by tumors are also considered. Comprised of 12 chapters, this volume begins with a detailed account of the vitamin D receptor, paying particular attention to its biochemical and physical properties as well as its mechanism of action. The discussion then turns to experimental discrimination between alternative mechanistic models for the receptor-mediated stimulation of adenylate cyclase; the role of microaggregation in hormone-receptor-effector interactions; and the biology and biochemistry of the Ah receptor. Subsequent chapters explore the interactions of animal viruses with cell surface receptors; insulin receptors; determination of the size of neurotransmitter receptors by radiation inactivation-target size analysis; and protein glycosylation and receptor-ligand interactions. This book will be a valuable resource for students and practitioners in fields ranging from cell biology and biochemistry to physiology, endocrinology, and pharmacology.

Autoren/Hrsg.

Conn, P. Michael

Inhaltsverzeichnis

1;Front Cover;1
2;The Receptors;4
3;Copyright Page;5
4;Table of Contents;6
5;Contributors;12
6;Preface;14
7;Contents of Previous Volume;16
8;CHAPTER 1. The Vitamin D Receptor;20
8.1;I. Introduction;20
8.2;II. Discovery of the Receptor Proteins for 1,25-(OH)2D3;24
8.3;III. Biochemical and Physical Properties of the 1,25-(OH)2D3 Receptor;25
8.4;IV. Mechanism of Action of the 1,25-(OH)2D3 Receptor;31
8.5;V. Regulation of Receptor Number;37
8.6;VI. Purification of Chick Intestinal 1,25-(OH)2D3 Receptor;40
8.7;VII. Antibodies to Chick Intestinal 1,25-(OH)2D3 Receptor;44
8.8;VIII. Conclusions;45
8.9;References;46
9;CHAPTER 2. On Experimental Discrimination between Alternative Mechanistic Models for the Receptor-Mediated Stimulation of Adenylate Cyclase;56
9.1;I. Introduction;56
9.2;II. General Assumptions, Definitions, and Nomenclature;57
9.3;III. Is the Receptor a Catalyst or a Reactant?;60
9.4;IV. What Are the Functional Relationships between R, N, and C, and How Are They Affected by Agonist and Guanine Nucleotide?;65
9.5;V. Summary;73
9.6;References;74
10;CHAPTER 3. The Role of Microaggregation in Hormone-Receptor-Effector Interactions;76
10.1;I. Introduction;76
10.2;II. The GnRH Receptor;77
10.3;III. Evidence That Microaggregation Plays a Key Role in Other Antibody-Receptor-Effector Systems;83
10.4;IV. The Adenylate Cyclase System;90
10.5;V. Receptor-Effector Interactions Revisited;97
10.6;References;101
11;CHAPTER 4. The Ah Receptor: A Biochemical and Biologic Perspective;108
11.1;I. Introduction;108
11.2;II. Biochemical Characterization of the Ah Receptor;114
11.3;III. Biology of the Ah Receptor;120
11.4;IV. Mechanism(s) of Action: Cell Biology and Biochemical Approaches;127
11.5;V. Concluding Comments;139
11.6;References;140
12;CHAPTER 5. Interactions of Animal Viruses with Cell Surface Receptors;150
12.1;I. Introduction;150
12.2;II. Viral Attachment Proteins;151
12.3;III. Identification of Cell Surface Receptors;169
12.4;IV. Cell Surface Receptor Determinants of Animal Viruses;175
12.5;V. Role of Receptors in Viral Penetration;198
12.6;VI. Role of Viral Receptors in Host Range and Tissue Tropism;207
12.7;VII. Summary and Future Perspectives;216
12.8;References;217
13;CHAPTER 6. Studies on Insulin Receptors: Implications for Insulin Action;240
13.1;I. Introduction;240
13.2;II. Receptor Purification;241
13.3;III. Receptor Structure;242
13.4;IV. Glycoprotein Nature of the Insulin Receptor;244
13.5;V. Tyrosyl Phosphorylation;244
13.6;VI. Interrelations of the Insulin Receptor to Other Receptor Structures;245
13.7;VII. Receptor-Mediated Internalization and Degradation of Insulin;247
13.8;VIII. Down-Regulation;248
13.9;IX. Possible Involvement of Cytoskeletal Elements in Insulin Action;249
13.10;X. Receptor Valence;250
13.11;XI. Nonlinear Scatchard Plots;250
13.12;XII. Possible Role for Receptor Cross-Linking and for Non-Insulin-Binding Regulatory Glycoprotein;251
13.13;XIII. Transmembrane Signaling;253
13.14;References;255
14;CHAPTER 7. Size of Neurotransmitter Receptors as Determined by Radiation Inactivation-Target Size Analysis;264
14.1;I. Introduction;264
14.2;II. Radiation Inactivation-Target Size Analysis;265
14.3;III. Conclusions and Avenues for Further Study;294
14.4;References;295
15;CHAPTER 8. a2-Adrenergic Receptors: Apparent Interaction with Multiple Effector Systems;300
15.1;I. Historical Perspective and Introduction;300
15.2;II. Identification of a-Adrenergic Receptors;302
15.3;III. Properties of a2-Adrenergic Receptors Linked to Inhibition of Adenylate Cyclase;302
15.4;IV. Postulated Mechanisms by Which a2-Adrenergic Agents Inhibit Adenylate Cyclase Activity;311
15.5;V. Postulated Mechanisms by Which a2-Adrenergic Agents Elicit Physiological Effects;313
15.6;VI. Summary;318
15.7;References;319
16;CHAPTER 9. Protein Glycosylation and Receptor–Ligand Interactions;326
16.1;I. Introduction;326
16.2;II. Carbohydrates in Determining Specificity;327
16.3;III. General Features of Glycoproteins;328
16.4;IV. Structural Organization of the Glycoprotein Hormones;329
16.5;V. Biological Properties of the Glycoprotein Hormones;330
16.6;VI. Experimental Approaches to Studying the Role of Carbohydrate Units;332
16.7;VII. Role of Carbohydrate in the Recognition and Uptake of Glycoprotein by Cells;337
16.8;VIII. Glycoprotein Hormone-Receptor Interactions;339
16.9;IX. Role of Carbohydrate in Receptor Assembly and Function;352
16.10;X. Concluding Remarks;354
16.11;References;355
17;CHAPTER 10. Role of Steroid Hormone Receptors in Development and Puberty;360
17.1;I. Introduction;360
17.2;II. Glucocorticoid Receptors;362
17.3;III. Androgen Receptors;364
17.4;IV. Estrogen Receptors;367
17.5;V. Progesterone Receptors;370
17.6;VI. Gonadal Steroid Receptors and Brain Development;372
17.7;VII. Puberty;376
17.8;References;379
18;CHAPTER 11. Functions and Regulation of Cell Surface Receptors in Cultured Leydig Tumor Cells;386
18.1;I. Introduction;387
18.2;II. Differentiated Function of MA-10 Cells;387
18.3;III. Interaction of hCG with MA-10 Cells;389
18.4;IV. Interaction of mEGF with MA-10 Cells;402
18.5;V. Mechanisms Involved in the Homologous and Heterologous Down-Regulation of hCG Receptors Are Different;405
18.6;VI. Regulation of Steroidogenic Responses by mEGF and hCG;407
18.7;VII. Regulation of Steroidogenic Responses by Cholesterol Availability: The Role of Low-Density Lipoprotein;411
18.8;VIII. Summary;415
18.9;References;417
19;CHAPTER 12. Somatostatin Receptors in Endocrine Cells;420
19.1;I. Introduction;420
19.2;II. Characteristics of Somatostatin Binding in Endocrine Cells;422
19.3;III. Modulation of Somatostatin Binding by Hormones and Secretagogues;426
19.4;IV. Biological Significance of Somatostatin-Receptor Translocation;435
19.5;References;439
20;Index;442