Conn Cell Culture

1. Auflage 2013
ISBN: 978-1-4832-6218-5
Verlag: Elsevier Science & Techn.
Format: EPUB
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 440 Seiten

Methods in Neurosciences, Volume 3: Quantitative and Qualitative Microscopy is a collection of papers that deals with microscopic techniques in statistical measures. This volume describes microscopy using sophisticated stains and dyes to advance observation of tests and experiments. Section I describes autoradiography including micro chemical methods, high-resolution autoradiography, and single- or double-label quantitative autoradiography for use in imaging of brain activity patterns or determining cerebral physiology. Section II discusses the quantification of structures through statistical and computational methods including dynamic video imaging technology. Section III explains the use of tracers, toxins, or dyes in tracing neuronal connections. One paper addresses the use of small injections of axonally transported fluorescent tracers. Section IV explains staining technology such as using the silver impregnation method for frozen sections of human nervous tissue that are gathered from tissues preserved in formalin. Section V addresses freezing techniques and those using freeze-fracture methods in neurobiology. The text also discusses cryoprotection and other freezing methods to control ice crystals found in fixed or unfixed brain tissues. Section VI presents the combined and high-resolution methods in polarization microscopy and microscopic investigations. Cellular biologists, micro-chemists, and scientific researchers in the field of micro- and cellular biology will appreciate this book.

Autoren/Hrsg.

Conn, P. Michael

Leseproben

Contributors to Volume 2

Ruben Adler(10),     Retinal Degenerations Research Center, Retinal Degenerations Laboratories, Wilmer Eye Institute, The Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205

Efrain C. Azmitia(17),     Department of Biology, New York University, New York, New York 10003

Barbara A. Bennett(21),     Department of Physiology and Pharmacology, The Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, North Carolina 27103

Michael E. Berens(16),     Brain Tumor Research Center, Department of Neurosurgery, School of Medicine, University of California, San Francisco, San Francisco, California 94143

Rolf Bjerkvig(15),     Department of Pathology, The Gade Institute, University of Bergen, Haukeland Hospital, N-5021 Bergen, Norway

Ira B. Black(1),     Division of Developmental Neurology, Department of Neurology and Neuroscience, Cornell University Medical College and The New York Hospital, New York, New York 10021

Liane Bologa(11),     Institute of Anatomy, University of Fribourg, CH-1700 Fribourg, Switzerland

E. Peter Bosch(4),     Division of Neurochemistry and Neurobiology, Department of Neurology, The University of Iowa, College of Medicine, Iowa City, Iowa 52242

Jane E. Bottenstein(5),     Marine Biomedical Institute and Department of Human Biological Chemistry & Genetics, The University of Texas Medical Branch at Galveston, Galveston, Texas 77550

P. Michael Conn(13),     Department of Pharmacology, The University of Iowa, College of Medicine, Iowa City, Iowa 52242

Stanley M. Crain(6),     Departments of Neuroscience and of Physiology and Biophysics, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461

Cheryl F. Dreyfus(1),     Division of Developmental Neurology, Department of Neurology and Neuroscience, Cornell University Medical College and The New York Hospital, New York, New York 10021

Gary R. Dutton(7),     Department of Pharmacology, The University of Iowa, College of Medicine, Iowa City, Iowa 52242

Laura Facci(2),     Department of CNS Research, Fidia Research Laboratories, 35031 Abano Terme, Italy

Annie Faivre-Bauman(23),     Groupe de Neuroendocrinologie Cellulaire et Moléculaire URA 1115, Collège de France, Centre National de la Recherche Scientifique (CNRS), 75231 Paris Cédex 05, France

Muhammad Farooq(12),     Department of Neurology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461

Paul C. Goldsmith(16),     Reproductive Endocrinology Center, Department of Obstetrics, Gynecology and Reproductive Sciences, School of Medicine, University of California, San Francisco, San Francisco, California 94143

James R. Hansen(13),     Department of Pediatrics, The University of Iowa, College of Medicine, Iowa City, Iowa 52242

Norbert Herschkowitz(11),     Department of Pediatrics, University of Bern, CH-3010 Bern, Switzerland

Samuel F. Hunter(5),     Department of Pharmacology & Toxicology, The University of Texas Medical Branch at Galveston, Galveston, Texas 77550

Marcus Jacobson(25),     Department of Anatomy, University of Utah Medical School, Salt Lake City, Utah 84132

Richard M. Kris(22),     Rorer Biotechnology Incorporated, King of Prussia, Pennsylvania 19406

Kinya Kuriyama(8),     Department of Pharmacology, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoij, Kamikyo-Ku, Kyoto 602, Japan

Ole Didrik Laerum(15),     Department of Pathology, The Gade Institute, University of Bergen, Haukeland Hospital, N-5021 Bergen, Norway

Mei Lee(22),     Department of Biochemistry, The George Washington University Medical Center, Washington, D.C. 20037

Alberta Leon(2),     Department of CNS Research, Fidia Research Laboratories, 35031 Abano Terme, Italy

Jon E. Levine(20),     Section of Biological Sciences, Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208

Ramon Lim(4),     Division of Neurochemistry and Neurobiology, Department of Neurology, The University of Iowa, College of Medicine, Iowa City, Iowa 52242

Daria Milani(2),     Department of CNS Research, Fidia Research Laboratories, 35031 Abano Terme, Italy

Terry W. Moody(22),     Department of Biochemistry, The George Washington University Medical Center, Washington, D.C. 20037

Mariana Morris(21),     Department of Physiology and Pharmacology, The Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, North Carolina 27103

Sean Murphy(3),     Department of Pharmacology, The University of Iowa, College of Medicine, Iowa City, Iowa 52242

William T. Norton(12),     Department of Neurology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461

Seitaro Ohkuma(8),     Department of Pharmacology, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoij, Kamikyo-Ku, Kyoto 602, Japan

John S. Ramsdell(19),     Division of Molecular and Cellular Endocrinology, Department of Anatomy and Cell Biology, Medical University of South Carolina, Charleston, South Carolina 29425

Russell P. Saneto(9),     Department of Cell Biology and Anatomy, Oregon Health Sciences University, Portland, Oregon 97201

Abraham Shahar(14),     Section of Electron Microscopy, Department of Virology, Israel Institute for Biological Research (IIBR), 70450 Ness-Ziona, Israel

Stephen D. Skaper(2),     Department of CNS Research, Fidia Research Laboratories, 35031 Abano Terme, Italy

Julie Staley(22),     Department of Biochemistry, The George Washington University Medical Center, Washington, D.C. 20037

Gunther S. Stent(24),     Department of Molecular & Cell Biology, University of California, Berkeley, Berkeley, California 94720

Frank J. Strobl(20),     Section of Biological Sciences, Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208

Duncan K. Stuart(24),     Department of Molecular & Cell Biology, University of California, Berkeley, Berkeley, California 94720

David K. Sundberg(21),     Department...