Clos Leishmania

Methods and Protocols
Erscheinungsjahr 2019
ISBN: 978-1-4939-9210-2
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1971, 370 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume explores the latest techniques used to study Leishmania and other trypanosomatidae. Chapters in this book cover a wide range of topics such as cultivation of axenic amastigote forms; phylogeny and comparative genomics; and in vitro and in vivo infection models that look at hosts ranging from cell lines to mammalian and arthropode hosts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and cutting-edge, Leishmania: Methods and Protocols is a valuable guide for both established and novice researchers in the Leishmania field. This book will also be useful to researchers working with other protozoa of the Order Trypanosomatide.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Clos, Joachim

Inhaltsverzeichnis

Host-Free Systems for Differentiation of Axenic Leishmania.- Phylogenetic Studies.- A Guide to Next Generation Sequence Analysis of Leishmania Genomes.- Quantitative RNA Analysis using RNA Seq.- Ribosome Profiling in Trypanosomatids.- Cosmid Library Construction and Functional Cloning.- Cos-Seq, A High Throughput Gain of Function Screen for Drug Resistance Studies in Leishmania.- Gene Replacement by Homologous Recombination.- LeishGEdit: A Method for Rapid Gene Knockout and Tagging using CRISPR-Cas9.- DiCre-Based Inducible Disruption of Leishmania Genes.- DiCre-Based Inducible Gene Expression.- Generation of Bone Marrow-Derived Macrophages for In Vitro Infection Experiments.- Quantification of Intracellular Leishmania spp. Using Real-Time Quantitative PCR (qPCR).- In Vitro Infections of Macrophage-Like Cell Lines with Leishmania infantum for Drug Screening.- Quantification of Parasite Loads by Automated Microscopic Image Analysis.- Quantification of Leishmania Parasites in Murine Models of Visceral Infection.- Syrian Hamster as an Advanced Experimental Model for Visceral Leishmaniasis.- Experimental Cutaneous Leishmaniasis: Mouse Models for Resolution of Inflammation versus Chronicity of Disease.- Establishment, Maintenance of Phlemotomus spp. In the Laboratory, and Infection with Leishmania spp.