Chang / Ohi The Mitotic Spindle

Methods and Protocols
1. Auflage 2016
ISBN: 978-1-4939-3542-0
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1413, 424 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume includes a series of
protocols focused on mitotic spindle assembly and function. The methods covered
in this book feature a broad range of techniques from basic microscopy to the
study of spindle physiologies relevant to cancer. These methods can be applied
to diverse model systems that range from the cell-free
Xenopus
egg
extract system to the moss
Physcomitrella patens
, in an effort to
demonstrate the key contributions made by researchers using multiple model
organisms. Chapters in
The Mitotic Spindle: Methods and Protocols 
integrate cutting-edge technologies that have only become available due to the
cross-disciplinary efforts, such as ATP analogue sensitive inhibition of
mitotic kinases. Written in the highly successful
Methods in Molecular Biology

series format, chapters include introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips ontroubleshooting and avoiding
known pitfalls.

Thorough and informative,
The
Mitotic Spindle: Methods and Protocols,
is a valuable resource for
researchers who are new to mitosis or are already experts in the field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Chang, Paul

Ohi, Ryoma

Inhaltsverzeichnis

Using Fluorescence
Microscopy to Study Mitosis.- Using Photoactivatable
GFP to Study Microtubule Dynamics and Chromosome Segregation.- Purification and Fluorescent Labeling
of Tubulin from
Xenopus Laevis
Egg Extracts.- Measuring
the Effects of Microtubule-Associated Proteins on Microtubule Dynamics
In Vitro.-
Imaging
and Quantifying the Dynamics of ?-Tubulin at Microtubule Minus Ends in Mitotic Spindles.- Visualizing and Analyzing Branching Microtubule Nucleation Using Meiotic
Xenopus
Egg Extracts and TIRF Microscopy.- Encapsulation
of
Xenopus
Egg and Embryo Extract Spindle
Assembly Reactions in Synthetic Cell-Like Compartments
with Tunable Size.-
In Vitro
Kinetochore Assembly.- Biochemical
and Structural Analysis of Kinetochore Histone-Fold Complexes.- Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells.- Studying
Kinetochores
In Vivo
Using FLIM-FRET.- Purification of Fluorescently Labeled

Saccharomyces Cerevisiae
Spindle Pole
Bodies.- A Cell-Free
System for Real-Time Analyses of Centriole Disengagement and Centriole to Centrosome
Conversion.- Assays
to Study Mitotic Centrosome and Spindle Pole Assembly and Regulation.- Analyzing
Spindle Positioning Dynamics in Cultured Cells.- Quantification of Mitotic Chromosome Alignment.- Imaging Mitosis
in the Moss
Physcomitrella Patens.- 
Small Molecule
Approach to Study the Function of Mitotic Kinesins.- Identification
and Characterization of Mitotic Spindle-Localized Transcripts.- Probing Mitosis
by Manipulating the Interactions of Mitotic Regulator Proteins Using Rapamycin-Inducible
Dimerization.- Studying
Kinetochore Kinases.- Engineering
and Functional Analysis of Mitotic Kinases through Chemical Genetics.- Using Cell
Culture Models of Centrosome Amplification to Study Centrosome Clustering in Cancer.- Generation
and Purification of Tetraploid Cells.- Anti-Microtubule
Drugs.