Capek DNA Engineered Noble Metal Nanoparticles

Fundamentals and State-of-the-Art of Nanobiotechnology
1. Auflage 2015
ISBN: 978-1-119-12092-6
Verlag: John Wiley & Sons
Format: EPUB
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 672 Seiten, E-Book

Reihe: Advance Materials Series

There is a growing interest in the use of nanoparticles modifiedwith DNAs, viruses, peptides and proteins for the rational designof nanostructured functional materials and their use in biosensorapplications. The challenge is to control the organization ofbiomolecules on nanoparticles while retaining their biologicalactivity as potential chemical and gene therapeutics. Thesenoble metal nanoparticles/biomolecules conjugates have specificproperties and therefore they are attractive materials fornanotechnology in biochemistry and medicine.
In this book, the author review work performed dealing with theDNA structure and functionalities, interactions between DNA, noblemetal nanoparticles, surface active agents, solvents and otheradditives. Particular attention is given to how the DNA'schain length and the DNA conformation affect the interaction andstructure of the nanoconjugates and nanostructures that are formed.Also discussed are the recent advances in the preparation,characterization, and applications of noble metal nanoparticlesthat are conjugated with DNA aptamers and oligomers. The advantagesand disadvantages of functionalized nanoparticles through variousdetection modes are highlighted, including colorimetry,fluorescence, electrochemistry, SPR, and, mass spectrometry for thedetection of small molecules and biomolecules. The functionalizednoble metal nanoparticles are selective and sensitive for theanalytes, showing their great potential in biosensing. Furthermore,this book reviews recent progress in the area of DNA-noble metalnanoparticles based artificial nanostructures, that is, thepreparation, collective properties, and applications of variousDNA-based nanostructures are also described.

Autoren/Hrsg.

Capek, Ignac

Inhaltsverzeichnis

Acknowledgement ix
Preface xi
1 Introduction 1
2 Nucleic Acids 29
2.1 DNA/RNA Basics 29
2.2 Aptamers, Telomers and Oligonucleotides 50
2.3 Techniques and Approaches 63
2.4 DNA-Based Molecular Nanomachines 90
2.5 Peptide Nucleic Acid 104
2.6 Nanobiotechnology 112
3 Noble Metal Nanoparticles 121
3.1 Preparation and Modification 121
3.2 Optical and Physical Properties 137
3.3 Conjugates 144
4 DNA-Based Conjugates 149
4.1 General 149
4.2 Condensation 152
4.3 Conjugates 170
5 DNA-Noble Metal Nanoparticle Conjugates 173
5.1 General Approaches 173
5.2 DNA Monomers and Oligomers 178
5.2.1 Gold Nanoparticles 178
5.2.2 Silver Nanoparticles 207
5.3 Hybridization and Denaturation 224
5.4 DNA Biotemplates 273
6 DNA-Gold Nanoparticle Conjugates 281
6.1 DNA-Gold Zero-Dimensional Nanoparticle Conjugates 281
6.2 DNA One-Dimensional Gold Nanoparticle Conjugates 295
7 PNA-Noble Metal Nanoparticles Conjugates 315
7.1 PNA-Gold Nanoparticle Conjugates 315
7.2 PNA-Silver Nanoparticle Conjugates 328
8 DNA-Silver Nanoparticles Conjugates 335
9 The Structure of DNA-Noble Metal Nanoparticles Conjugates 345
9.1 Configuration of DNA-Noble Metal Nanoparticles Conjugates 345
9.2 Stabilization of DNA Conjugates 358
9.3 Nanostructures and Nanoconstructs 369
9.4 Colorimetric and Sensing Assays 385
10 Photochemical and Photophysical Events 403
10.1 Noble Metal Nanoparticles 403
10.2 DNA Nucleobases 406
10.3 DNA/PNA 417
10.4 DNA-Dyes Conjugates 429
10.5 DNA-AuNP-Dye Conjugates 443
10.6 DNA-Gold Nanoparticle Conjugates 455
10.7 DNA-AgNPs 465
10.8 Hot Gold Nanoparticles 475
11 Nanoparticle Therapeutics 481
11.1 Biodecorated Nanoparticle-Based Therapies 481
11.2 Photothermal Therapy 489
11.3 Cells 495
11.4 Gene Therapy 499
11.5 Blood Fluid Effect 505
11.6 Other Application Approaches 507
12 Conclusion 515
Nomenclature 533
DNA Entities 545
Vocabulary and Definitions 549
References 564
Index