Cannell Natural Products Isolation

Erscheinungsjahr 2008
ISBN: 978-1-59259-256-2
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 480 Seiten

Reihe: Methods in Biotechnology

Researchers from both industry and academia present in detail techniques for the extraction and isolation of natural products of all classes. These experienced experimentalists describe a wide array of methodologies, including basic chromatography, ion-exchange methods, HPLC, countercurrent and planar chromatography, SFE, and crystallization. They also address the specific problems involved in working with plants, marine organisms, water-soluble compounds, scale-up, dereplication and partial identification, and follow-up of a natural product lead. For the less-experienced they offer hands-on advice-using real examples-about how to approach an extraction in general, when and why to apply a particular technique, how to carry out the methods described, and how to modify conditions according to the compound being isolated.

Zielgruppe

Research

Autoren/Hrsg.

Cannell, Richard J. P.

Inhaltsverzeichnis

How to Approach the Isolation of a Natural Product.- Initial Extraction and Product Capture.- Supercritical Fluid Methods.- Isolation by Low-Pressure Column Chromatography.- Isolation by Ion-Exchange Methods.- Isolation by Preparative HPLC.- Isolation by Planar Chromatography.- Separation by High-Speed Countercurrent Chromatography.- Crystallization and Final Stages of Purification.- Dereplication and Partial Identification of Natural Products.- Purification of Water-Soluble Natural Products.- Special Problems with the Extraction of Plants.- Isolation of Marine Natural Products.- Scale-Up of Natural Products Isolation.- Follow-Up of Natural Product Isolation.