Cancela / Maggioli | Fasciola hepatica | E-Book | sack.de
E-Book

E-Book, Englisch, Band 2137, 235 Seiten, eBook

Reihe: Methods in Molecular Biology

Cancela / Maggioli Fasciola hepatica

Methods and Protocols

E-Book, Englisch, Band 2137, 235 Seiten, eBook

Reihe: Methods in Molecular Biology

ISBN: 978-1-0716-0475-5
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark



This volume described basic and advanced protocols to study
F. hepatica
parasite biology. Chapters guide readers through protocols on different developmental stages of
F. hepatica,
characterize tegumental, secreted proteins, spatial and temporal gene expression, immunological effects of ESP on macrophages, eosinophil apoptosis, macrophages alternative activation, toll-like receptor interactions, obtain peritoneal and splenic dendritic cells of mice, and protocols to detect
F. hepatica
albendazole resistance. Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and cutting-edge,
Fasciola hepatica: Methods and Protocols
provides different topics in areas such a biochemistry,immunology, molecular biology, microscopy, vaccinology in order to extend the interest in this book to research community working with
Fasciola hepatica
and related trematodes.
Cancela / Maggioli Fasciola hepatica jetzt bestellen!

Zielgruppe


Professional/practitioner

Weitere Infos & Material


Maintenance of Life Cycle at the Laboratory.- Microscopical Techniques to Analyse the Hepatic and Peritoneal Changes Caused by 
Fasciola hepatica 
Infection.- Isolation of Secreted and Tegumental Surface Proteins from 
Fasciola hepatica.- 
Isolation and Analysis of 
Fasciola hepatica
 Extracellular Vesicles.- Cloning and Heterologous Expression of Protein-Coding Sequences in
 Escherichia coli.- 
Gene Silencing in the Liver Fluke 
Fasciola hepatica
: RNA Interference.- Analysis of Gene Expression in 
Fasciola hepatica
 Juveniles and Adults by In Situ Hybridization.- Evasion of Host Immunity during 
Fasciola hepatica
 Infection.- Immunomodulatory Effect of 
Fasciola hepatica
 Excretory-secretory Products on Macrophages.- Study of Eosinophil Apoptosis Induced by 
Fasciola hepatica
 Excretory-secretory Products.- Purification of native Fasciola hepatica Fatty Acid Binding Protein and the Induction of Alternative Activation of Human Macrophages.- Possible Role for Toll-Like Receptors in Interaction of Fasciola hepatica Excretory-Secretory Products with Human Monocyte Cell Line.- Evaluation of the Immune Regulatory Properties of Dendritic Cells during 
Fasciola hepatica
 Infection.- Design of a Peptide–carrier Vaccine Based on the Highly Immunogenic 
Fasciola hepatica
 Leucine Aminopeptidase.- Liver Fluke Vaccine Assessment in Cattle.- Testing Albendazole Resistance in 
Fasciola hepatica.- 
Drug Targets: Screening for Small Molecules that Inhibit 
Fasciola hepatica 
Enzymes.


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