Cancela / Maggioli Fasciola hepatica

Methods and Protocols
Erscheinungsjahr 2020
ISBN: 978-1-0716-0475-5
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 2137, 235 Seiten, eBook

Reihe: Methods in Molecular Biology

This volume described basic and advanced protocols to study F. hepatica parasite biology. Chapters guide readers through protocols on different developmental stages of F. hepatica, characterize tegumental, secreted proteins, spatial and temporal gene expression, immunological effects of ESP on macrophages, eosinophil apoptosis, macrophages alternative activation, toll-like receptor interactions, obtain peritoneal and splenic dendritic cells of mice, and protocols to detect F. hepatica albendazole resistance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Fasciola hepatica: Methods and Protocols provides different topics in areas such a biochemistry,immunology, molecular biology, microscopy, vaccinology in order to extend the interest in this book to research community working with Fasciola hepatica and related trematodes.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Cancela, Martin

Maggioli, Gabriela

Inhaltsverzeichnis

Maintenance of Life Cycle at the Laboratory.- Microscopical Techniques to Analyse the Hepatic and Peritoneal Changes Caused by  Fasciola hepatica  Infection.- Isolation of Secreted and Tegumental Surface Proteins from  Fasciola hepatica.-  Isolation and Analysis of  Fasciola hepatica  Extracellular Vesicles.- Cloning and Heterologous Expression of Protein-Coding Sequences in  Escherichia coli.-  Gene Silencing in the Liver Fluke  Fasciola hepatica : RNA Interference.- Analysis of Gene Expression in  Fasciola hepatica  Juveniles and Adults by In Situ Hybridization.- Evasion of Host Immunity during  Fasciola hepatica  Infection.- Immunomodulatory Effect of  Fasciola hepatica  Excretory-secretory Products on Macrophages.- Study of Eosinophil Apoptosis Induced by  Fasciola hepatica  Excretory-secretory Products.- Purification of native Fasciola hepatica Fatty Acid Binding Protein and the Induction of Alternative Activation of Human Macrophages.- Possible Role for Toll-Like Receptors in Interaction of Fasciola hepatica Excretory-Secretory Products with Human Monocyte Cell Line.- Evaluation of the Immune Regulatory Properties of Dendritic Cells during  Fasciola hepatica  Infection.- Design of a Peptide–carrier Vaccine Based on the Highly Immunogenic  Fasciola hepatica  Leucine Aminopeptidase.- Liver Fluke Vaccine Assessment in Cattle.- Testing Albendazole Resistance in  Fasciola hepatica.-  Drug Targets: Screening for Small Molecules that Inhibit  Fasciola hepatica  Enzymes.