Cadenas / Packer Nitric Oxide, Part D

1. Auflage 2002
ISBN: 978-0-08-049703-7
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band Volume 359, 514 Seiten, Web PDF

Reihe: Methods in Enzymology

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. - Presents information on nitrosothiols and nitric oxide in cell signaling - Details nitric oxide and mitochondrial functions - Explains nitric oxide synthases

Autoren/Hrsg.

Cadenas, Enrique

Packer, Lester

Inhaltsverzeichnis

1;Cover;1
2;Table of Contents;6
3;Contributors to Volume 359;10
4;Preface;16
5;Volume in Series;18
6;Section I: Detection of Nitric Oxide;40
6.1;Chapter 1. Electron Paramagnetic Resonance for Quantitation of Nitric Oxide in Aqueous Solutions;42
6.2;Chapter 2. In Vitro Detection of Nitric Oxide and Nitroxyl by Electron Paramagnetic Resonance;57
6.3;Chapter 3. Iron Dithiocarbamate as Spin Trap for Nitric Oxide Detection: Pitfalls and Successes;66
6.4;Chapter 4. Advanced Spin Trapping of Vascular Nitric Oxide Using Colloid Iron Diethyldithiocarbamate;81
6.5;Chapter 5. Simultaneous Detection of pO2 and NO by Electron Paramagnetic Resonance;91
6.6;Chapter 6. Electron Paramagnetic Resonance Studies Nitric Oxide in Living Mice;105
6.7;Chapter 7. Measuring End Products of Nitric Oxide in Vivo;114
6.8;Chapter 8. Guide for the Use of Nitric Oxide (NO) Donors as Probes of the Chemistry of NO and Related Redox Species in Biological Systems;123
6.9;Chapter 9. Amperometric Measurement of Nitric Oxide Using a Polydemethylsiloxane-Coated Electrode;144
6.10;Chapter 10. Nitric Oxide Monitoring in Hippocampal Brain Slices Using Electrochemical Methods;150
6.11;Chapter 11. Electrochemical Detection of Physiological Nitric Oxide: Materials and Methods;164
6.12;Chapter 12. Detection of Nitric Oxide Production by Fluorescent Indicators;173
6.13;Chapter 13. Specific Analysis of Nitrate and Nitrite by Gas Chromatography/Mass Spectrometry;187
6.14;Chapter 14. Griess Method for Nitrite Measurement of Aqueous and Protein-Containing Samples;197
6.15;Chapter 15. Gas-Phase Oxidation and Disproportionation of Nitric Oxide;207
6.16;Chapter 16. Detection and Quantification of Nitric Oxide (NO) Synthase-Independent Generation of NO;219
6.17;Chapter 17. Nitric Oxide-Dependent Vasodilation in Human Subjects;225
6.18;Chapter 18. Antioxidant and Diffusion Properties of Nitric Oxide in Low-Density Lipoprotein;239
6.19;Chapter 19. Measurements of Redox Control of Nitric Oxide Bioavailability;248
7;Section II: Nitrosothiols and Nitric Oxide in Cell Signaling;256
7.1;Chapter 20. Reactions of S-Nitrosothiols with L-Ascorbic Acid in Aqueous Solution;258
7.2;Chapter 21. Nitrosothiols and S-Nitrosohemoglobin;268
7.3;Chapter 22. Nitrosothiol Processing by Platelets;277
7.4;Chapter 23. Nitric Oxide-Activated Glutathione Sepharose;284
7.5;Chapter 24. Measurement of Protein Nitration and S-Nitrosothiol Formation in Biology and Medicine;295
7.6;Chapter 25. S-Glutathionylation of NF-.B Subunit p50;307
7.7;Chapter 26. Intercellular Signaling Mediated by Nitric Oxide in Human Glioblastoma Cells;319
7.8;Chapter 27. L-Arginine Metabolism in Trypanosoma cruzi in the Regulation of Programmed Cell Death;325
8;Section III: Nitric Oxide and Mitochondrial Functions;342
8.1;Chapter 28. Measurement of Mitochondrial Respiratory Thresholds and the Control of Respiration by Nitric Oxide;344
8.2;Chapter 29. Determination of Nitric Oxide-Induced Effects on Tissue Levels of Glutathione and Mitochondrial Membrane Potential;358
8.3;Chapter 30. Pharmacological Regulation of Mitochondrial Nitric Oxide Synthase;367
8.4;Chapter 31. Characterization of Mitochondrial Nitric Oxide Synthase;378
9;Section IV: Peroxynitrite;390
9.1;Chapter 32. Peroxynitrite Formation from Biochemical and Cellular Fluxes of Nitric Oxide and Superoxide;392
9.2;Chapter 33. Evaluation of Antioxidant Activity Using Peroxynitrite as a Source of Radicals;405
9.3;Chapter 34. Peroxynitrite Reactions with Heme and Heme- Thiolate (P450) Proteins;418
9.4;Chapter 35. Gas Chromatography/Mass Spectrometry Assay for 3-Nitrotyrosine;429
9.5;Chapter 36. Quantitation and Localization of Tyrosine Nitration in Proteins;438
10;Section V: Nitric Oxide Synthases;450
10.1;Chapter 37. Neuronal Nitric Oxide Synthases in Brain and Extraneural Tissues;452
10.2;Chapter 38. Visualization and Distribution of Neuronal Nitric Oxide Synthase-Containing Neurons;463
10.3;Chapter 39. Quantitative Measurement of Endothelial Constitutive Nitric Oxide Synthase;472
10.4;Chapter 40. Quantitation of Expressed Message for Inducible Nitric Oxide Synthase;484
10.5;Chapter 41. Inducible Nitric Oxide Synthase Knockout Mouse and Low-Density Lipoprotein Oxidation;491
10.6;Chapter 42. Nitric Oxide and Resolution of Inflammation;498
11;Author Index;506
12;Subject Index;540