DNA and RNA Profiling in Human Blood

Methods and Protocols
2009
ISBN: 978-1-62703-913-0
Verlag: Humana Press

Buch, Englisch, Band 496, 341 Seiten, Previously published in hardcover, Format (B × H): 193 mm x 260 mm, Gewicht: 743 g

Reihe: Methods in Molecular Biology

Blood samples have consistently proven to be a key source of genetic material for a wide variety of diagnostic or research purposes. In DNA and RNA Profiling in Human Blood: Methods and Protocols, leading international experts contribute both established and recently developed protocols for complex and high-throughput DNA and RNA profiling. Divided into two thorough sections, the volume concentrates on DNA profiling for blood cell antigens through methods on high-throughput multiplex approaches and SNP typing, along with RNA profiling in blood cells addressing certain blood cell types such as platelets, reticulocytes, and megakaryocytes. Written in the highly successful Methods in Molecular Biology™ series format, all of the chapters include brief introductions on the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as the Notes section which highlights tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, DNA and RNA Profiling in Human Blood: Methods and Protocols is an ideal guide to the molecular profiling approaches that have opened up this broad field of research and have shown great promise in the further identifying of disease markers in blood.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Bugert, Peter

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Molekulare Medizin, Zellbiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Humangenetik
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Hämatologie, Transfusionsmedizin
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Biomedizin, Medizinische Forschung, Klinische Studien
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

DNA Profiling for Blood Cell Antigens.- PCR–ELISA for High-Throughput Blood Group Genotyping.- Single Base Extension in Multiplex Blood Group Genotyping.- Real-Time PCR Assays for High-Throughput Blood Group Genotyping.- Real-Time PCR Assays for High-Throughput Human Platelet Antigen Typing.- Multiplex ABO Genotyping by Minisequencing.- Multiplex Genotyping for Thrombophilia-Associated SNPs by Universal Bead Arrays.- Pyrosequencing of Toll-Like Receptor Polymorphisms of Functional Relevance.- Multiplex HLA-Typing by Pyrosequencing.- High-Throughput Multiplex HLA-Typing by Ligase Detection Reaction (LDR) and Universal Array (UA) Approach.- Medium- to High-Throughput SNP Genotyping Using VeraCode Microbeads.- The Use of Maternal Plasma for Prenatal RhD Blood Group Genotyping.- RNA Profiling in Blood Cells.- Nanoliter High-Throughput PCR for DNA and RNA Profiling.- RNA Stabilization of Peripheral Blood and Profiling by Bead Chip Analysis.- RNA Profiling in Peripheral Blood Cells by Fluorescent Differential Display PCR.- cDNA Amplification by SMART-PCR and Suppression Subtractive Hybridization (SSH)-PCR.- Transcript Profiling of Human Platelets Using Microarray and Serial Analysis of Gene Expression (SAGE).- Genome-Wide Platelet RNA Profiling in Clinical Samples.- PCR-Based Amplification of Platelet mRNA Sequences Obtained From Small-Scale Platelet Samples.- MicroRNA Profiling of Megakaryocytes.- Serial Analysis of Gene Expression Adapted for Downsized Extracts (SAGE/SADE) Analysis in Reticulocytes.- Real-Time PCR Analysis for Blood Cell Lineage Specific Markers.- Monitoring the Immune Response Using Real-Time PCR.