Bosio / Gerstmayer Microarrays in Inflammation

2008
ISBN: 978-3-7643-8334-3
Verlag: Springer
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 232 Seiten, eBook

Reihe: Progress in Inflammation Research

This book summarizes the most advanced technical aspects covering all steps for a thorough application of microarrays to inflammation topics – from sample generation to data analysis. In addition selected examples of successful applications of microarrays in major fields of inflammation research are presented. The book will help a researcher or clinician to plan, perform and analyze or to critically review microarray experiments related to inflammation research.

Zielgruppe

Research

Autoren/Hrsg.

Bosio, Andreas

Gerstmayer, Bernhard

Inhaltsverzeichnis

Microarray and inflammation: An introduction.- Microarray and inflammation: An introduction.- Methods and protocols for the generation of gene expression profiles in inflammation research.- Whole blood.- Separation of whole blood cells and its impact on gene expression.- Skin and skin models.- Adipose tissue.- Samples isolated by flow cytometry.- Magnetic cell sorting.- T7 based amplification protocols.- Amplification of cDNA from single or rare cells by global PCR (exponential amplification).- Selected applications of microarrays in inflammation research.- Gene expression patterns in asthma.- Gene expression profiling in neurological and neuroinflammatory diseases.- Aspects of gene expression in B cell lymphomas.- The genes behind rheumatology.- miRNA patterns in hematopoietic malignancies.- Keep it simple: microarray cross-platform comparison without statistics.- Microarrays in drug development: regulatory perspective.- Outlook.- Complementary microarray technologies.