Histochemistry of Single Molecules

Methods and Protocols
1. Auflage 2017
ISBN: 978-1-4939-6787-2
Verlag: Springer

Buch, Englisch, Band 1560, 341 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 8196 g

Reihe: Methods in Molecular Biology

This detailed volume explores numerous histochemical techniques through a series of lab-tested protocols for the detection of specific molecules or metabolic processes, both at light and electron microscopy. More in detail, the book is divided into six sections covering a variety of chemical targets.  It begins with a section on vital histochemistry and continues with chapters on histochemistry as it relates to lectins, proteins, lipids, DNA and RNA, as well as plants. The volume also contains four overview chapters on vital histochemistry, lectin histochemistry, and DNA  fluorochromes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Histochemistry of Single Molecules: Methods and Protocols aims to effectively help scientists in very different research fields to elucidate biological issues though a unique approach to molecular biology in situ.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Biggiogera, Marco

Pellicciari, Carlo

Fachgebiete

• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

Single Cell Cytochemistry Illustrated by the Demonstration of Glucose-6-Phosphate Dehydrogenase Deficiency in Erythrocytes.- Autofluorescence Spectroscopy for Monitoring Metabolism in Animal Cells and Tissues.- Enzyme-Histochemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in the Liver Biliary Tree.- Histochemical Demonstration of Tripeptidyl Aminopeptidase I.- Enzyme Histochemistry for Functional Histology in Invertebrates.- Lectin Histochemistry: Historical Perspectives, State of the Art, and the Future.- Isolation of Viable Glycosylation-Specific Cell Populations for Further In Vitro or In Vivo Analysis Using Lectin-Coated Magnetic Beads.- Lectin Histochemistry for Metastasizing and Non-Metastasizing Cancer Cells.- The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium.- Heat-Induced Antigen Retrieval in Immunohistochemistry: Mechanisms and Applications.- Detecting Neuronal Differentiation Markers in Newborn Cells of the Adult Brain.- Characterizing Satellite Cells and Myogenic Progenitors During Skeletal Muscle Regeneration.- Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin Fixed and Paraffin Embedded Tissue.- Tissue Fixation and Processing for the Histological Identification of Lipids.- Staining Methods for Normal and Regenerative Myelin in the Nervous System.- Nile Red Staining of Neutral Lipids in Yeast.- Staining of Lipid Droplets with Monodansylpentane.- Fluorochromes for DNA Staining and Quantitation.- Osmium Ammine for Staining DNA at Electron Microscopy.- DNA Labeling at Electron Microscopy.- Visualizing RNA at Electron Microscopy by Terbium Citrate.- Two-Tailed Comet Assay (2T-Comet): Simultaneous Detection of DNA Single and Double Strand Breaks.- Detection of Endogenous Nuclear Proteins in Plant Cells: Localizing Nuclear Matrix Constituent Proteins (NMCPs), the Plant Analogues of Lamins.- Histochemical Analysis of Plant Secretory Structures.- A Histochemical Technique for the Detection of Annonaceous Acetogenins.