Bier Electrophoresis

Theory, Methods, and Applications
1. Auflage 2013
ISBN: 978-1-4832-7429-4
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 572 Seiten, Web PDF

Electrophoresis: Theory, Methods, and Applications, Volume II focuses on the contributions of electrophoresis in the advancement of knowledge on proteins, as well as in the fields of biochemistry, physiology, and medicine. The selection first offers information on the interpretation of electrophoretic mobilities, including theories for other models, electrophoresis of polyelectrolytes, and theory for a rigid spherical particle. The text then takes a look at primary protein structures and nomenclature and identification of the normal human serum proteins. Discussions focus on principles of nomenclature of the serum constituents; methods of identification of an isolated antigen; principal methods used to study serum proteins; separation of mixtures of peptides and amino acids by high-voltage electrophoresis; and methods for determining the primary structure of proteins. The publication elaborates on inheritance of protein variation in human serum and antibodies and myeloma proteins. Topics include products of enzymic digestion, products of reduction, naturally occurring fragments, genetic variation, and variations detected in human serum proteins. The manuscript then examines lymph and cerebrospinal fluid, electrophoresis of gastrointestinal secretions, and high resolution techniques. The selection is a dependable source of data for readers interested in electrophoresis.

Autoren/Hrsg.

Bier, Milan

Inhaltsverzeichnis

1;Front Cover;1
2;Electrophoresis: Theory, Methods, and Applications;4
3;Copyright Page;5
4;Table of Contents;10
5;Contributors;6
6;Preface;8
7;Contents of Volume I;14
8;Introduction;16
9;Chapter 1.
The Interpretation of Electrophoretic Mobilities;20
9.1;I. Introduction;20
9.2;II. General Considerations;22
9.3;III. Theory for a Rigid Spherical Particle;25
9.4;IV. Theories for Other Models;40
9.5;V. Electrophoresis of Polyelectrolytes;59
9.6;REFERENCES;68
10;Chapter 2. Primary Protein Structures;72
10.1;I. General Conclusions from Present Knowledge of Primary Protein Structure;72
10.2;II. Methods for Determining the Primary Structure of Proteins;83
10.3;III. Separation of Mixtures of Peptides and Amino Acids by High-Voltage Electrophoresis;106
10.4;REFERENCES;117
11;Chapter 3.
Nomenclature and Identification of the Normal Human Serum Proteins;128
11.1;I. Introduction;129
11.2;II. Principal Methods Used to Study Serum Proteins;130
11.3;III. Methods of Identification of an Isolated Antigen;133
11.4;IV. Principles of Nomenclature of the Serum Constituents;139
11.5;V. The Immunoglobulins;141
11.6;VI. .- and ß2-Proteins Other Than Immunoglobulins;146
11.7;VII. The ß1-Globulins;148
11.8;VIII. The a2-GIobulins;154
11.9;IX. The a1-GIobulins;161
11.10;X. Serum Albumin;163
11.11;XI. The ao-Globulins;164
11.12;XII. The Prealbumins (p-Proteins);165
11.13;XIII. The Lipoproteins;166
11.14;XIV. The Serum Enzymes;169
11.15;REFERENCES;171
12;Chapter 4.
Inheritance of Protein Variation in Human Serum;176
12.1;I. Genetic Variation;176
12.2;II. Variations Detected in Human Serum Proteins;185
12.3;ACKNOWLEDGMENT;225
12.4;REFERENCES;225
13;Chapter 5.
Antibodies and Myeloma Proteins;232
13.1;I. Introduction;232
13.2;II. Whole Proteins;236
13.3;III. Products of Enzymic Digestion;241
13.4;IV. Products of Reduction;246
13.5;V. Naturally Occurring Fragments;251
13.6;VI. Concluding Remarks;255
13.7;REFERENCES;256
14;Chapter 6.
Lymph and Cerebrospinal Fluid;260
14.1;I. Introduction;260
14.2;II. Lymph;264
14.3;III. Cerebrospinal Fluid;296
14.4;REFERENCES;323
15;Chapter 7.
Electrophoresis of Gastrointestinal Secretions;330
15.1;I. Introduction;330
15.2;II. Saliva;332
15.3;III. Gastric Juice;344
15.4;IV. Pancreatic Juice;376
15.5;V. Duodenal and Jejunal Juice and Meconium;379
15.6;VI. Bile;381
15.7;REFERENCES;390
16;Chapter 8. High Resolution TechniquesHi;398
16.1;I. Introduction;398
16.2;II. Analytical Methods;399
16.3;III. Preparative Procedures;426
16.4;IV. Photography of Gels;430
16.5;V. Difficulties and Limitations;431
16.6;REFERENCES;435
17;Chapter 9. Preparative Electrophoresis;442
17.1;I. Introduction;442
17.2;II. Electrophoresis in Homogeneous Media;444
17.3;III. Zone Electrophoresis on Supporting Media;456
17.4;IV. Continuous Zone Electrophoresis;468
17.5;REFERENCES;484
18;Chapter 10. Automatic Density Gradient Electrophoresis;492
18.1;I. Introduction;492
18.2;II. Principles and Methods;494
18.3;III. The Automatic Electrophoresis Apparatus;497
18.4;REFERENCES;509
19;Chapter 11. Electrophoresis in Semifluid Films;512
19.1;I. Introduction;512
19.2;II. Procedures and Control of Critical Factors;513
19.3;III. Applications;516
19.4;IV. Detection and Quantitation by Far Ultraviolet Photometry;523
19.5;V. The Spectrophor;525
19.6;REFERENCES;527
20;Author Index;530
21;Subject Index;564