Beta / Martinez-Torres Cell Motility and Chemotaxis

Methods and Protocols
Erscheinungsjahr 2024
ISBN: 978-1-0716-4023-4
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 222 Seiten

Reihe: Methods in Molecular Biology

This volume explores the latest experimental and computer-based methods used to study the field of motility research. The chapters in this book cover topics such as methods to investigate motility and chemotaxis in a range of systems including macrophages and ; ways in which electric fields impact motility and taxis; recently developed computer-based methods for studying pseudopod-driven locomotion of amoeboid cells; and software tools for kymograph-based analysis of cell contour dynamics. Written in the highly successful series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, is a valuable resource for researchers looking to expand their knowledge of motility-related research across different disciplines.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Beta, Carsten

Martinez-Torres, Cristina

Inhaltsverzeichnis

Chemotaxis Assay of Bone Marrow-Derived Macrophages.- Methods and Protocol for Single Cell Motility Assays under Topological Constraints.- Imaging-Based Analysis of Cell-Cell Contact Dependent Migration in Dictyostelium.- A Mutant of Dictyostelium discoideum, KI-Cell, As a Model of Collective Cell Migration Independent of Chemotaxis.- Using Multiphoton Intra-Vital Microscopy to Study Neutrophil Transmigration and Blood-Brain Barrier Permeability in a Mouse Model of Herpes Simplex Virus Encephalitis.- An In Vivo Metastasis Model using Genotype-Defined Tumor Organoids.- Different Morphotypes of Physarum polycephalum as Models for Chemotaxis and Locomotion.- Flagella and Cell Body Staining of Bacteria with Fluorescent Dyes.- Re-Purposing Proximity-Dependent Protein Labelling (BioID2) for Protein Interaction Mapping in E. coli.- Electrotaxis of Dictyostelium discoideum, Migration in an Electric Field.- Electric Cell-substrate Impedance Sensing as a Tool to Characterize Wound Healing Dynamics.- Chemotaxis of Large Multinucleate Cells of Dictyostelium Produced by Electric-Pulse Induced Fusion.- A Hands-On Guide to AmoePy: A Python-Based Software Package to Analyze Cell Migration Data.- Pseudopod Tracking and Statistics during Cell Movement in Buffer and Chemotaxis.- Generation of a Virtual Cell using a Phase Field Approach to Model Amoeboid Crawling.