Analytical Techniques in Biochemistry

1. Auflage 2020
ISBN: 978-1-0716-0136-5
Verlag: Springer US

Buch, Englisch, 126 Seiten, Format (B × H): 178 mm x 254 mm, Gewicht: 306 g

Reihe: Springer Protocols Handbooks

This book provides a comprehensive overview of the major biochemical analytical techniques, with detailed descriptions of the instrumentation and applications. The contributions, which each focus on a specific technique, are based on a thorough review and analysis of the current literature as well as the authors’ experiences in the lab. 

Divided into nine parts, the book provides insights into basic separation techniques like sedimentation, filtration and centrifugation, as well as analytical techniques such as spectrophotometry, chromatography, electrophoresis, immuno-techniques, radioactivity and microscopy. 

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Basha, Mahin

Fachgebiete

• Naturwissenschaften Biowissenschaften Biowissenschaften
• Naturwissenschaften Chemie Analytische Chemie Instrumentelle Analytik, Chromatographie
• Naturwissenschaften Chemie Analytische Chemie Magnetresonanz
• Technische Wissenschaften Sonstige Technologien | Angewandte Technik Medizintechnik, Biomedizintechnik
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Medizintechnik, Biomedizintechnik, Medizinische Werkstoffe

Inhaltsverzeichnis

1 Sedimentation 1.1 Gravity sedimentation 1.2 Zone Settling &Compression2 Filtration              2.1 Microfiltration 2.2 Ultrafiltration3 Centrifugation 3.1 Two basic modes of centrifugation 3.2 Instrument Design4 Colorimetry and spectrophotometer (spectrophotometry) 4.1 Colorimetry 4.2 Spectrophotometry5 Chromatography 5.1 Partition Chromatography          5.1.1  Paper chromatography          5.1.2 Thin Layer Chromatography          5.1.3 HPLC                 5.1.4  Gas Chromatography          5.1.5 Gel filtration chromatography6 Electrophoresis
6.1 Paper Electrophoresis
6.2 SDS-Polyacrylamide Gel Electrophoresis
6.3 Immunoelectrophoresis
7 Immuno-techniques
7.1 Immunodiffusion
7.2 Immuno-fluorescence 
7.3 Immunoassay: Enzyme Linked Immunosorbent Assay (ELISA) 
7.4 Radioimmunoassay (RIA)
7.5 Western blotting or Immunoblotting 
7.6 Southern Blotting
7.7 Northern Blot
8. Radioactivity
9. Microscopy

Über Autor(innen)

Dr. Mahin Basha, MSc (Biochemistry), M.Tech (Industrial Biotechnology) and PhD in Chemical Engineering, has over seven years of experience in the field of academic research and teaching at various respected institutions. He is currently serving as an Assistant Professor of Biochemistry, P.M. Sayeed Calicut University Centre, Affiliated to University of Calicut, Kerala, India, one of the India's most prestigious institutes. Dr. Basha's research has been published in a number of leading, international journals and he has presented his work at scientific meetings around the globe.