Barnard / Aston Spermatogenesis

Methods and Protocols
Erscheinungsjahr 2012
ISBN: 978-1-62703-038-0
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 927, 554 Seiten, eBook

Reihe: Methods in Molecular Biology

Deficiencies in sperm function are usually the result of spermatogenic defects. Spermatogenesis is a biologically complex and essential process during which spermatogonia undergo meiotic recombination, reduction of the genome to a haploid state, and extensive cellular modifications that result in a motile cell capable of traversing the female reproductive tract, withstanding various potential assaults to viability, and finally successfully fertilizing a mature oocyte to give rise to an embryo. Defects in any step of spermatogenesis or spermatogenesis can lead to male infertility, a disease that affects approximately 5-7% of the population.  Spermiogenesis and Spermatogenesis: Methods and Protocols  details protocols used in the study of spermatogenesis, clinical analytical protocols, and basic techniques used in clinical andrology laboratories, such as obtaining accurate results for a sperm count, and advanced procedures, such as genome-wide genetic study tools and evaluation of nuclear proteins. Written in the successful  Methods in Molecular Biology™  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible,  Spermiogenesis and Spermatogenesis: Methods and Protocols  is unique in its breadth, and will be a useful reference for clinicians and researchers alike.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Barnard, Lori

Aston, Kenneth I.

Inhaltsverzeichnis

Methods For Sperm Concentration Determination.- Methods of Sperm Vitality Assessment.- The Hypo-osmotic Swelling Test for Evaluation of Sperm Membrane Integrity.- Sperm Morphology Classification: A Rational Method For Schemes Adopted By The World Health Organization.- Sperm Morphology Assessment Using Strict (Tygerberg) Criteria.- Methods For Direct And Indirect Antisperm Antibody Testing.- Manual Methods For Sperm Motility Assessment.- Computer-Aided Sperm Analysis (CASA) Of Sperm Motility And Hyperactivation.- The Hemizona Assay For Assessment Of Sperm Function.- The Sperm Penetration Assay for the Assessment of Fertilization Capacity.- Methods For The Assessment Of Sperm Capacitation And Acrosome Reaction Excluding The Sperm Penetration Assay.- Sperm DNA Fragmentation Analysis Using the TUNEL Assay.- Sperm DNA Damage Measured by Comet Assay.- Sperm Chromatin Structure Assay (SCSA®).- Sperm Aneuploidy Testing Using Fluorescence In Situ Hybridization (FISH).- Flow Cytometric Methods For Sperm Assessment.- Human Y Chromosome Microdeletion Analysis By PCR Multiplex Protocols Identifying Only Clinically Relevant AZF Microdeletions.- Sperm Cryopreservation Methods.- Density Gradient Separation Of Sperm For Artificial Insemination.- Recovery, Isolation, Identification and Preparation of Spermatozoa from Human Testis.- Enhancement of Sperm Motility Using Pentoxifylline and Platelet-Activating Factor.- Intracytoplasmic Morphology Selected Sperm Injection.- Sperm Selection For ICSI Using Annexin V.- Sperm Selection for ICSI Using the Hyaluronic Acid Binding Assay.- Sperm Selection Based On Electrostatic Charge.- Sex-Sorting Sperm Using Flow Cytometry/Cell Sorting.- Assessment of Spermatogenesis Through Staging of Seminiferous Tubules.- Immunohistochemical Approaches for the Study of Spermatogenesis.- Ultrastructural Analysis of Testicular Tissue and Semen by Transmission and Scanning Electron Microscopy.- Assessment Of Oxidative Stress In Sperm And Semen.-Improved Chemiluminescence Assay for Measuring Antioxidant Capacity of Seminal Plasma.- Methods of Sperm DNA Extraction for Genetic and Epigenetic Studies.- Isolating mRNA and Small Noncoding RNAs From Human Sperm.- A Review Of Genome-Wide Approaches To Study The Genetic Basis For Spermatogenic Defects.- Methods For The Analysis Of The Sperm Proteome.- Methodology of Aniline Blue Staining Of Chromatin and the Assessment of the Associated Nuclear and Cytoplasmic Attributes in Human Sperm.- Isolation of Sperm Nuclei and Nuclear Matrices from the Mouse, and Other Rodents.- Extraction and Analysis of Human Sperm Protamine 1 / Protamine 2 Ratio Using Acid Gel Electrophoresis.- Analysis of Gene-Specific and Genome-Wide Sperm DNA Methylation.- Evaluating the Localization and DNA Binding Complexity of Histones in Mature Sperm.- In Vitro Spermatogenesis Using An Organ Culture Technique.- Testicular Tissue Grafting and Male Germ Cell Transplantation.- Transgenic Modification of Spermatogonial Stem Cells Using Lentiviral Vectors.- Methods for Sperm-Mediated Gene Transfer.- Phenotypic Assessment of Male Fertility Status in Transgenic Animal Models.