Bacterial Pathogenesis, Part C: Identification, Regulation and Function of Virulence Factors

1. Auflage 2002
ISBN: 978-0-08-056957-4
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band Volume 358, 527 Seiten, Web PDF

Reihe: Methods in Enzymology

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant todaytruly an essential publication for researchers in all fields of life sciences.Key Features* Presents alternatives to mammalian model systems* Discusses virulence and essential gene identification* Defines global gene expression

Autoren/Hrsg.

Inhaltsverzeichnis

1;Cover;1
2;Table of Contents;6
3;Contributors to Volume 358;10
4;Preface;16
5;Volume in Series;18
6;Section I: Alternatives to Mammalian Model Systems;40
6.1;Chapter 1. Use of Plant and Insect Hosts to Model Bacterial Pathogenesis;42
6.2;Chapter 2. Identification of Host and Pathogen Factors Involved in Virulence Using Caenorhabditis elegans;52
6.3;Chapter 3. Goldfish as an Animal Model System for Mycobacterial Infection;68
7;Section II: Virulence and Essential Gene Identification;80
7.1;Chapter 4. Green Fluorescent Protein as a Marker for Conditional Gene Expression in Bacterial Cells;82
7.2;Chapter 5. Genetic Methods for Deciphering Virulence Determinants of Mycobacterium tuberculosis;106
7.3;Chapter 6. Analysis of Gene Function in Bacterial Pathogens by GAMBIT;139
7.4;Chapter 7. Microbial Gene Expression Elucidated by Selective Capture of Transcribed Sequences (SCOTS);147
7.5;Chapter 8. Identification of Essential Genes in Staphylococcus aureus Using Inducible Antisense RNA;162
7.6;Chapter 9. Transposomes: A System for Identifying Genes Involved in Bacterial Pathogenesis;167
7.7;Chapter 10. Functional Screening of Bacterial Genome for Virulence Genes by Transposon Footprinting;180
7.8;Chapter 11. Use of LexA-Based System to Identify Protein–Protein Interactions in Vivo;192
8;Section III: Global Gene Expression: Microarrays and Proteomics;202
8.1;Chapter 12. Borrelia burgdorferi Gene Expression Profiling with Membrane-Based Arrays;204
8.2;Chapter 13. Transcript Profiling of Escherichia coli Using High-Density DNA Microarrays;216
8.3;Chapter 14. Neisseria Microarrays;227
8.4;Chapter 15. Acquisition and Archiving of Information for Bacterial Proteomics: From Sample Preparation to Database;246
8.5;Chapter 16. Proteomic Analysis of pH-Dependent Stress Responses in Escherichia coli and Helicobacter pylori Using Two-Dimensional Gel Electrophoresis;267
8.6;Chapter 17. Mycobacterial Proteomes;281
8.7;Chapter 18. Proteomic Analysis of Response to Acid in Listeria Monocytogenes;295
8.8;Chapter 19. Proteome Analysis of Chlamydia pneumoniae;316
8.9;Chapter 20. Enrichment and Proteomic Analysis of Low- Abundance Bacterial Proteins;327
8.10;Chapter 21. Immunoproteome of Helicobacter pylori;346
9;Section IV: Bacterial Perturbations of Eukaryotic Cell Cycle and Apoptosis;356
9.1;Chapter 22. Helicobacter pylori and Apoptosis;358
9.2;Chapter 23. Modulation of Apoptosis during Infection with Chlamydia;373
9.3;Chapter 24. Measurement of Pore Formation by Contact- Dependent Type III Protein Secretion Systems;384
9.4;Chapter 25. Interaction of Enteropathogenic Escherichia coli with Red Blood Cell Monolayers;389
10;Section V: Bacterial Modification or Exploitation of Eukaryotic Signal Transduction;396
10.1;Chapter 26. GAP Activity of Yersinia YopE;398
10.2;Chapter 27. Tyrosine Phosphorylation of Eukaryotic Proteins and Translocated Intimin Receptor by Enteropathogenic Escherichia coli;409
11;Section VI: Type III Secretion Systems;422
11.1;Chapter 28. Purification and Detection of Shigella Type III ;424
11.2;Chapter 29. Analysis of Salmonella Invasion Protein–Peptidoglycan Interactions;432
12;Section VII: Quorum Sensing and Gene Regulation;450
12.1;Chapter 30. RP4-Based Plasmids for Conjugation between Escherichia coli and Members of the Vibrionaceae;452
12.2;Chapter 31. Role of Autoinducers in Gene Regulation and Virulence of Pseudomonas aeruginosa;466
12.3;Chapter 32. Quorum-Sensing System of Agrobacterium Plasmids: Analysis and Utility;491
13;AUTHOR INDEX;524
14;SUBJECT INDEX;554