Augustin Methods in Endothelial Cell Biology

2004
ISBN: 978-3-642-18725-4
Verlag: Springer
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 383 Seiten, eBook

Reihe: Springer Lab Manual

Endothelial cell biology has developed into a vibrant discipline and has become a critical instrument to study several disease processes on the cellular and molecular level. It is now widely recognized that dysfunctions of normal endothelial cell homeostasis are involved in some of the most important human diseases, including ischemic heart diseases, hypertension, atherosclerosis, tumors, diabetes, arthritis, and inflammation. Further, the increasing importance and recognition of the field of vascular biology in general requires in vitro and in vivo techniques in order to address the complex questions.

is a comprehensive practical "how-to"-guide summarizing the most relevant established techniques as well as a number of new emerging techniques. Easy-to-follow reliable protocols provide a useful lab bench resource for the experienced researcher and newcomer to the field.

Zielgruppe

Research

Autoren/Hrsg.

Augustin, Hellmut G.

Inhaltsverzeichnis

Part I: Isolation and Culture of Endothelial Cells Chapter 1 Isolation, Purification, and Culture of Human Micro- and Macrovascular Endothelial Cells
Nancy Laurens andVictor W.M. van Hinsbergh Chapter 2 Isolation of Mouse Cardiac Microvascular Endothelial Cells
Ralf P. Brandes, Stefani Beer, and Alexandra Aicher Chapter 3 Isolation,Culture, and Differentiation of Circulating Endothelial Precursor Cells

Carmen Urbich and Stefanie Dimmeler Chapter 4 Embryonic Stem Cell-Derived Endothelial Cells

Jun Yamashita and Shin-Ichi Nishikawa Chapter 5 Detection, Isolation, and Culture Of Lymphatic Endothelial Cells

Taija Mäkinen and Kari Alitalo Chapter 6 Three-Dimensional Spheroid Culture of Endothelial Cells

Thomas Korff Chapter 7 Immortalization of Endothelial Cells

Yvonne Reiss and Friedemann Kiefer Chapter 8 Viral Gene Transfer Into Endothelial Cells

Christoph Volpers and Stefan Kochanek Part II: Functional Assays in Vitro Chapter 9 Leukocyte Adhesion to Endothelium in Vitro Under Shear Stress

Olaf Zille, Markus Hammel, and Rupert Hallmann Chapter 10 Application of Biomechanical Forces on Endothelial Cells

Andreas Schubert and Henning Morawietz Chapter 11 Endothelial Cell Permeability Assays in Culture

Maria Grazia Lampugnani and Elisabetta Dejana Chapter 12 Three-Dimensional in Vitro Angiogenesis Assays

Thomas Korff Chapter 13 Rat Aortic Ring Assay of Angiogenesis

Roberto F. Nicosia and Wen-Hui Zhu Chapter 14 Endothelial Cell Chemotaxis and Chemokinesis Assays

Hellmut G.Augustin Chapter 15 FACS Analysis of Endothelial Cells

Yvonne Reiss and Britta Engelhardt Chapter 16 Application of the RNA Interference (RNAi) Technology to Angiogenesis Research

Sung-Suk chae, Ji-Hye Paik, Jonathan Shubert-Coleman, Henry Furneaux, andTimothy Hla Part III: Functional Assays in Vivo Chapter 17 Murine Bone Marrow Transplantation Models that Enable the Study of EPC Recruitment

Toshinori Murayama,Oren M. Tepper, and Takayuki Asahara Chapter 18 Balloon Denudation of Blood Vessels

Volkhard Lindner Chapter 19 Peripheral Hind Limb Ischemia Models

Stephan Schirmer, Imo Hoefer, and Ivo Buschmann Chapter 20 In Vivo Matrigel Angiogenesis Assays

Tony Passaniti and Michele I.Vitolo Chapter 21 Analysis of Vascular Permeability in vivo

Katja Issbrücker,Hugo H.Marti, and Matthias Clauss Chapter 22 Validating the Manipulation of Endothelial Cell signaling in Vivo

John S. Rudge and Gavin Thurston Chapter 23 Chick Chorioallantoic Membrane Assay: Growth Factor and Tumor-induced Angiogenesis and Lymphangiogenesis

Martin Hagedorn and Jörg Wilting Chapter 24 Corneal Angiogenesis Assay

Lucia Morbidelli, Silvia Cantara, and Marina Ziche Part IV: Detection of Endothelial Cells Chapter 25 Basic Blood Vessel Immunohistochemistry

Cynthia Obobodozie and Jens Kroll Chapter 26 Double-Staining Immunohistochemistry of Blood Vessels

Valentin Goede Chapter 27 In Situ Hybridization Analysis of Vascular Endothelium

Regina Heidenreich and Georg Breier Chapter 28 High-Resolution in Situ Confocal Analysis of Endothelial Cells

Holger Gerhardt and Christer Betsholtz Chapter 29 Whole Mount Analysis of the Embryonic Vasculature

Jody J.Haigh and Andras Nagy Chapter 30 Methods for Visualizing Intact Blood Vessels

Gavin Thurston and Danielle Jean-Guillaume Chapter 31 Intravital Multi-Fluorescence Microscopy

Mohammad Farhadi, Karsten Schwinn, and Peter Vajkoczy Chapter 32 Corrosion Cast Analysis of Blood Vessels

Valentin Djonov and Peter H.