Astatke RNA Amplification and Analysis

Methods and Protocols
Erscheinungsjahr 2024
ISBN: 978-1-0716-3918-4
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 473 Seiten

Reihe: Springer Protocols

This volume provides methods to analyze RNA and gain insight to a number of critical cellular functions. Chapters detail RNA isolation and enrichment, RNA amplification and detection, RNA sequencing, analysis of RNA modification, RNA delivery approaches, and analysis of RNA binding and catalytic activity. Written in the highly successful  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, aims to present researchers with cutting-edge technologies with detailed explanations of critical steps, while providing a clear understanding of the overall protocol. 

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Astatke, Mekbib

Inhaltsverzeichnis

RNA Isolation from Human Stem Cell Derived Retinal Organoids.- Next generation Sequencing Application a Systematic Approach for High quality RNA Isolation from Skeletal Muscles.- Ultrasound induced Enrichment of Ultra trace miRNA Biosensing in Nanoliter Samples.- Direct Detection of SARS CoV 2 RNA in Saliva with Colorimetric RT LAMP.- NASBA Coupled to Paper Microfluidics for RNA Detection.- Single cell Imaging of mRNA by Target RNA initiated RCA.- Detection of Foodborne RNA Viruses by Reverse Transcriptase Droplet Digital PCR.- Single molecule RNA Imaging in Live Cells with an Avidity based Fluorescent Light up Aptamer biRhoBAST.- Assessing MicroRNA Profiles from Low Concentration Extracellular Vesicle RNA Utilizing NanoString nCounter Technology.- Liquid and Solid Hybridization Methods to Detect RNAs.- Visualization of Individual RNA Molecules by Proximity Ligation based Chromogenic In Situ Hybridization Assay.- Quantum Dots Based Protocols for the Detection of RNAs.- RNA Analysis Using Immunoassay Detection Format.- Fluorescent Techniques for RNA Detection in Nanoparticles.- Sequencing 10X Genomics 3’ HT assay for Gene Expression.- Single Cell Sequencing of 3’ RNA Transcripts.- Short Read RNA Seq.- RNA Seq Data Analysis.- Bi clustering for Epi transcriptomic Co functional Analysis.- RNA Folding, Mutation and Detection.- Epitranscriptomic Mass Spectrometry.- Polyvinylamine and Its Derivative as Effective Carrier for Targeted Delivery of Small RNAs.- Delivery of mRNA with histidine-lysine peptides.- Plant Virus Like Particles for RNA Delivery.- Lipid-based Nanoparticles for RNA Delivery.- In vitro Amplification and Selection of Engineered RNase P ribozyme for Gene Targeting Applications.- Rapid Ribonuclease P Kinetics Measured by Stopped-Flow Fluorescence and Fluorescence Anisotropy.- Computational Design of Allosteric Ribozymes via Genetic Algorithms.