Buch, Englisch, Band 1208, 477 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 9149 g
Reihe: Methods in Molecular Biology
Buch, Englisch, Band 1208, 477 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 9149 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-4939-4640-2
Verlag: Springer
Advanced Protocols in Oxidative Stress III continues the thread of the first two books by covering technology ranging from a portable hand-held detector for remote analysis of antioxidant capacity to sophisticated technology such as shotgun lipidomics, mitochondrial imaging, nano sensors, fluorescent probes, chromatographic fingerprints, computational models and bio statistical applications. Several chapters have shown the effect of pro-oxidation and antioxidants as inflammatory mediators in signaling pathways leading from the initial stimulus to termination through redox cycles. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, Advanced Protocols in Oxidative Stress III offers to save investigators significant time and effort, allowing them to focus on their own personal topic of interest.
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Weitere Infos & Material
Part I: Reactive Oxygen and Nitrogen Techniques
1. Derivatization and Detection of Small Aliphatic and Lipid-Bound Carbonylated Lipid Peroxidation Products by ESI-MS
Ivana Milic and Maria Fedorova
2. Specific Imaging and Tracking of Mitochondria in Live Cells by a Photostable AIE Luminogen
Chris W.T. Leung, Yuning Hong, and Ben Zhong Tang
3. Analysis of Relationship Between Oxidized Phospholipid Structure and Interaction with the Class B Scavenger Receptors
Detao Gao, Larry W. Sayre, and Eugene A. Podrez
4. A Novel Gas Chromatographic Method for Determination of Malondialdehyde from Oxidized DNA
Takayuki Shibamoto
5. Nitric Oxide Availability as a Marker of Oxidative Stress
Dan Pierini and Nathan S. Bryan
6. Quantification of DNA Repair Capacity Towards Oxidatively Damaged DNA in Subcellular and Cellular Systems by a Non-Radioactive Cleavage Assay
Ingrit Hamann and Andrea Hartwig
7. Chiral Phase-HPLC Separation of Hydroperoxyoctadecenoic Acids and Their Biosynthesis by Fatty Acid Dioxygenases
Ernst H. Oliw and Anneli Wennman
8. Selenium as a Versatile Center in Fluorescence Probe for the Redox Cycle Between HClO Oxidative Stress and HS Repair
Zhangrong Lou, Peng Li, and Keli Han
9. Evaluation of Physical Integrity of Lipid Bilayer Under Oxidative Stress: Application of Fluorescence Microscopy and Digital Image Processing
Ran Liang, Jian-Ping Zhang, and Leif H. Skibsted
10. Electrochemical Detection of Glutathione S-Transferase: An Important Enzyme in the Cell Protective Mechanism Against Oxidative Stress
Manuel C. Martos-Maldonado, Juan M. Casas-Solvas, Antonio Vargas-Berenguel, and Luis García-Fuentes
11. Using Nanosensors for In Situ Monitoring and Measurement of Nitric Oxide and Peroxynitrite in a Single Cell
Tadeusz Malinski
Part II: Antioxidant Technology and Application
12. Curcuma longa Attenuates Carbon Tetrachloride-Induced Oxidative Stress in T Lymphocyte Subpopulations
H.A. Abu-Rizq, Mohamed H. Mansour, and Mohammad Afzal
13. Prolongation of the Lag Time Preceding Peroxidation of Serum Lipids: A Measure of Antioxidant Capacity
Ilya Pinchuk and Dov Lichtenberg
14. Identification of (Anti-Oxidative) Plants in Herbal Pharmaceutical Preparations and Dietary Supplements
Eric Deconinck, Deborah Custers, and Jacques Omer De Beer
15. Simultaneous Determination of Ascorbic Acid, Aminothiols, and Methionine in Biological Matrices Using Ion-Pairing RP-HPLC Coupled with Electrochemical Detector
Muhammad Imran Khan, Zafar Iqbal, and Abad Khan
16. Portable Nanoparticle Based Sensors for Antioxidant Analysis
Erica Sharpe and Silvana Andreescu
17. Fe-Fe Transformation Method: An Important Antioxidant Assay
Ilhami Gülçin
18. Antioxidant Activity Evaluation Involving Hemoglobin-Related Free Radical Reactivity
Augustin C. Mot, Cristina Bischin, Grigore Damian, and Radu Silaghi-Dumitrescu
19. Computational Studies on Conformation, Electron Density Distributions, and Antioxidant Properties of Anthocyanidins
Ricardo A. Mosquera, Laura Estévez, and Mercedes García-Bugarín
20. Automatic Flow Injection Analysis (FIA) Determination of Total Reducing Capacity in Serum and Urine Samples
Marcela A. Segundo, Ildikó V. Tóth, Luís M. Magalhães, and Salette Reis
21. Mass Spectrometry Detection of Isolevuglandin Adduction to Specific Protein Residues
Casey D. Charvet and Irina A. Pikuleva
22. Preoxiredoxin Family Members (Prx3 & Prx4) and Pregnancy Disorder (Recurrent Pregnancy Loss)
Behrouz Gharesi-Fard
23. Substituted Pyridoindoles as Biological Antioxidants: Drug Design, Chemical Synthesis, and Biological Activity
Lucia Kovacikova, Magdalena Majekova, and Milan Stefek
24. Measuring Antioxidant and Prooxidant Capacity Using the Crocin Bleaching Assay (CBA)
Stella A. Ordoudi and Maria Z. Tsimidou
25. Simultaneous Determination of the Endogenous Free a-Lipoic Acid and Dihydrolipoic Acid in Human Plasma and Erythrocytes by RP-HPLC Coupled with Electrochemical Detector
Muhammad Imran Khan, Zafar Iqbal, and Abad Khan
26. Aryl-Acetic and Cinnamic Acids as Lipoxygenase Inhibitors with Antioxidant, Anti-Inflammatory, and Anti-Cancer Activity
Dimitra Hadjipavlou-Litina and Eleni Pontiki
27. Liquid Chromatography and Mass Spectrometry for the Analysis of N-ß-Methylamino-L-Alanine with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate
W. Broc Glover, Steven A. Cohen, and Susan J. Murch
Part III: Gene Expression
28. Intracellular Distribution of Glutathionylated Proteins in Cultured Dermal Fibroblasts by Immunofluorescence
Stefania Petrini, Valentina D’Oria, and Fiorella Piemonte
29. New Analytical Strategies Applied to the Determination of Coenzyme Q10 in Biological Matrix
Contin Mario, Flor Sabrina, Martinefski Manuela, Lucangioli Silvia, and Tripodi Valeria
30. Binding and Cytotoxic Trafficking of Cholesterol Hydroperoxides by Sterol Carrier Protein-2
Albert W. Girotti and Tamas Kriska
Part IV: Biostatistics
31. A Toolkit for Clinical Statisticians to Fix Problems Based on Biomarker Measurements Subject to Instrumental Limitations: From Repeated Measurement Techniques to a Hybrid Pooled-Unpooled Design
Albert Vexler, Ge Tao, and Xiwei Chen
