Practical Handbook on Soil Protists

1. Auflage 2024
ISBN: 978-1-0716-3749-4
Verlag: Springer

Buch, Englisch, 246 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 620 g

Reihe: Springer Protocols Handbooks

This volume details a wide range of basic and advanced techniques associated with research on isolation of important soil protozoa, and identification. Chapters guide readers through calculations of soil protists, basic techniques for isolation, enumeration, enrichment, molecular techniques, and staining techniques.

Authoritative and cutting-edge, Practical Handbook on Soil Protists aims to ensure successful results in the further study of this vital field.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Amaresan, N.

Chandarana, Komal A.

Fachgebiete

• Naturwissenschaften Biowissenschaften Mikrobiologie
• Naturwissenschaften Agrarwissenschaften Agrarwissenschaften
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie

Inhaltsverzeichnis

Part I: Abundance and species richness calculation of protists from soil

1. Soil sampling, processing and storage

Jean C. V. Dutra and Maria C. P. Batitucci

2. Initial observation of protist from soil

Veysel Turan

3. Abundance calculation of naked and testate amoebae from soil

Urjita Sheth

4. Abundance calculation of mycophagous amoebae

Urjita Sheth

5. Abundance calculation of ciliates from soil

Urjita Sheth

6. Abundance calculation of flagellates from soil

Urjita Sheth

7. Methodology to study species diversity of naked amoeba and testate amoeba

Hetvi Naik, Sapna Chandwani, and Natarajan Amaresan

8. Non-flooded Petri plate method to study the species diversity of flagellates

Hetvi Naik, Sapna Chandwani and Natarajan Amaresan

Part II: Enumeration techniques

9. Enumeration of testate amoeba through direct count from soil

Komal A. Chandarana, Hetvi Naik, and Natarajan Amaresan

10. Most Probable Number (MPN) method for enumeration of soil protists

Jun Murase

Part III: Isolation of protist from soil

11. Standard solutions and media used for isolation of soil protists

Sapna Chandwani, Hetvi Naik and Natarajan Amaresan

12. Isolation techniques for amoeba

Jun Murase

13. Isolation of ciliates and flagellates from soil

Amol D Theng, Bhavana V Mohite, andSatish V Patil

14. Isolation and Enumeration of Mycophagous Protist

Amol D Theng, Bhavana V Mohite, andSatish V Patil

Part IV: Enrichment of soil protists on laboratory media

15. Media used for enrichment of soil protists

Komal A. Chandarana, and Natarajan Amaresan

16. Enrichment of naked amoebae species

 Sapna Chandwani, Hetvi Naik, and Natarajan Amaresan

17. Enrichment of ciliates and flagellates

 Sapna Chandwani, Hetvi Naik and Natarajan Amaresan

18. Enrichment of mycophagous protists

 Sapna Chandwani, Hetvi Naik and Natarajan Amaresan

19. Mono-axenic cultivation of protists

Komal A. Chandarana, and Natarajan Amaresan

20. Axenic cultivation of soil protists

 Sapna Chandwani, Hetvi Naik and Natarajan Amaresan

Part V: Identification techniques for soil protists

21. Classical wet mount method for observing live protists

Komal A. Chandarana, and Natarajan Amaresan

22. SEM: Sample Preparation, Fixation and Staining of Protists

Shraddha Saha

23. TEM: Sample Preparation, Fixation and Staining of Protists

Shraddha Saha

24. Protargol staining technique for identification of soil protists

Mehreen Shah and Sirajuddin Ahmed

25. FLUTAX Staining of cilliates

Swati Pateland Vimal Prajapati

26. DNA extraction from trophozoites and cysts using manual protocol

Kejal Gohil, Komal A. Chandarana, G. Jawahar, and Natarajan Amaresan

27. DNA isolation from single ciliate isolate

Swati Patel and Vimal Prajapati

28. Species identification through sequencing

Vimalkumar Prajapati, Swati Patel, Vaibhavkumar Mehta, and B. Z. Dholakiya

29. DNA Barcoding techniques for Protist

Amit Gamit and Dhruti Amin

30. Unequivocal identification through fluorescence in situ hybridization (FISH) technique

Jean C. V. Dutraand Maria C. P. Batitucci

Part VI: Other Protistan

31. Isolation, DNA extraction, amplification, and gel electrophoresis of single-celled non-marine foraminifera (Rhizaria)

Maria Holzmann

32. Isolation and cultivation and staining of Paramecium

Satish V. Patil, Sunil H. Koli, Bhavana B. Mohite, Jitendra D. Salunkhe, Amol Theng, and Atharv S Patil

33. Isolation and cultivation of Euglena

Satish V. Patil, Sunil H. Koli, Bhavana B. Mohite, and Narendra S. Salunkhe Atharv S Patil