Sperm Chromatin for the Researcher

A Practical Guide
2013
ISBN: 978-1-4614-8458-5
Verlag: Springer

Buch, Englisch, 438 Seiten, Format (B × H): 155 mm x 235 mm, Gewicht: 7677 g

This concise, truncated version of Zini and Agarwal's Sperm Chromatin: Biological and Clinical Applications in Male Infertility and Assisted Reproduction offers select, research-oriented chapters for a handy, more affordable, state-of-the-art resource. Sperm Chromatin for the Researcher addresses such vital issues as:

- Structure and function of human sperm chromatin

- Biological determinants of sperm chromatin damage

- Laboratory evaluation of sperm chromatin

- Protocols to measure sperm chromatin damage

Ideal for novice and experienced researchers alike, Sperm Chromatin for the Researcher contains all of the need-to-know information about these cutting-edge topics in reproductive medicine.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Agarwal, Ashok

Zini, Armand

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Urologie, Andrologie, Venerologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Gynäkologie, Geburtshilfe

Inhaltsverzeichnis

Part I: Human Sperm Chromatin: Structure and Function.- Sperm Chromatin: An Overview.- Sperm Nucleoproteins.- The Relationship Between Chromatin Structure and DNA Damage in Mammalian Spermatozoa.- Chromosome Positioning in Spermatozoa.- Sperm Mitochondrial DNA.- The Sperm Epigenome.- RNA Expression in Male Germ Cells During Spermatogenesis (Male Germ Cell Transcriptome).- Part II: Biological Determinants of Sperm Chromatin Damage.- Spermatogenesis: An Overview.- Role of Oxidative Stress in the Etiology of Sperm DNA Damage.- Abortive Apoptosis and Sperm Chromatin Damage.- Spermiogenesis in Sperm Genetic Integrity.- Part III: Laboratory Evaluation of Sperm Chromatin.- Sperm Chromatin Structure Assay (SCSA®): 30 Years of Experience with the SCSA®.- Sperm Chromatin Dispersion Test: Technical Aspects and Clinical Applications.- Basic and Clinical Aspects of Sperm Chromomycin A3 Assay.- Cytochemical Tests for Sperm Chromatin Maturity.- Acridine Orange Test for Assessment of Human Sperm DNA Integrity.- Laboratory Evaluation of Sperm Chromatin: TUNEL Assay.- Basic and Clinical Aspects of Sperm Comet Assay.- Assays Used in the Study of Sperm Nuclear Proteins.- Sperm Epigenetic Profile.- Clinical Significance of Sperm RNA.- Part IV: Protocols and Integrity Tests.- Sperm Chromatin Structure Assay (SCSA®).- Measurement of DNA Damage in Spermatozoa by TUNEL Assay.

Über Autor(innen)

Armand Zini, MD, Department of Surgery, Division of Urology, McGill University, Montreal, QC, Canada

Ashok Agarwal, PhD, HCLD (ABB), Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA