Agarwal / Gupta / Sharma Andrological Evaluation of Male Infertility

A Laboratory Guide
1. Auflage 2016
ISBN: 978-3-319-26797-5
Verlag: Springer International Publishing
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 215 Seiten

Reihe: Medicine (R0)

This state-of-the-art laboratory manual includes 20 clinical protocols used daily for the investigation of the infertile male, presented with easy to understand, step-by-step methodology. The protocols are arranged from routine to advanced laboratory procedures common to clinical practice, including computer-assisted semen analysis, sperm preparation for IUI by density gradient and swim-up, sperm cryopreservation, and sperm DNA fragmentation test by TUNEL method, among others. The methodology in each protocol follows best practice guidelines made clearer by professionally hand-drawn illustrations covering most of the important steps and equipment. The authors, hailing from the world-renowned Andrology Center at Cleveland Clinic, have over 50 years of combined first-hand experience in managing very busy diagnostic and research facilities in male infertility and andrology. The book will be an indispensable resource for thousands of laboratory technologists, clinicians and reproductive professionals (andrologists, embryologist, etc.) engaged in the diagnosis and management of infertile men around the world.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Agarwal, Ashok

Gupta, Sajal

Sharma, Rakesh

Inhaltsverzeichnis

Diagnostic Tests in
the Evaluation of Male Infertility.- Quality Management in
Andrology Laboratory.- Competency Assessment
in Andrology Laboratory.- Basic Semen Analysis.- Semen Analysis Using Hamilton-Thorn Casa.- Semen Analysis Using SCA (v5) For Casa.- Leukocytospermia Quantitation (ENDTZ) Test.- Eosin-Nigrosin Staining Procedure.- Sperm Morphology
Stain (Diff-Quik®).- Qualitative Seminal
Fructose.- Cytospin Procedure $
Nuclear Fast Red and Picroindigocarmine Stain Procedures For Azoospermia
Samples.- Hypo-Osmotic Swelling
Test (HOS).- Procedure for
Retrograde Ejaculate.- Sperm Preparation for
Intrauterine Insemination Using Pureception®.- Sperm Preparation for
Intrauterine Inseminvation (IUI) by Swim-Up Method.- Cryopreservation of
Client Depositor Semen.- Cryopreservation of
Client Depositor Semen Aspirate.- Cryopreservation of
Client Depositor Testicular Tissue.- Acrosome Reaction
Measurement.- Direct Spermmar
Antibody Test.- Reactive Oxygen
Species (ROS) Measurement.- Antioxidant
Measurement in Seminal Plasma by TAC Assay.- DNA Damage in
Spermatozoa (TUNEL) BD Accuri™ C6 Flow Cytometer.- Licensing and
Accreditation of the Andrology Laboratory.

Über Autor(innen)

Ashok Agarwal, PhD, HCLD (ABB), ELD (ACE), Andrology Center and Reproductive Tissue Bank, American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA 

Sajal Gupta, MD (Ob/Gyn), MSc (Embryology), TS (ABB), Andrology Center and Reproductive Tissue Bank, American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA 

Rakesh Sharma, PhD, Andrology Center and Reproductive Tissue Bank, American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, USA