Wolf Isotopes in Biology

1. Auflage 2013
ISBN: 978-1-4832-2574-6
Verlag: Elsevier Science & Techn.
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, 184 Seiten, Web PDF

Isotope is Biology is a six-chapter supplementary text that covers the properties and application of isotopes as labels or analytical tools in biological research. The first chapters deal with the physico-chemical properties and radioactivity of isotopes. These chapters also explore their synthesis, preparation, radiation decomposition, and decay of radioactivity. The succeeding chapter considers other aspects of isotopes, including their effect of health, disposal, spills, and laboratory use. Another chapter examines the chemical and biochemical behavior, natural abundance, and the chemical stability of isotopic compounds. The final chapters describe several isotopic methods, namely, isotope dilution, paper chromatography, and autoradiography, with emphasis on their application in biological studies. This book will be of value to biologists, and graduate and undergraduate biology students.

Autoren/Hrsg.

Wolf, George

Inhaltsverzeichnis

1;Front Cover;1
2;Isotopes in Biology;4
3;Copyright Page;5
4;Table of Contents;8
5;PREFACE;6
6;Introduction;12
7;Chapter I. Some Physics and Chemistry;17
7.1;1. THE STRUCTURE OF MATTER; ISOTOPES;17
7.2;2. RADIOACTIVITY;20
7.3;3. ENERGY OF RADIATION;21
7.4;4. DECAY OF RADIOACTIVITY;24
7.5;5. PRODUCTION OF ISOTOPES;26
7.6;6. MEASUREMENT OF RADIOACTIVITY AND OF STABLE ISOTOPE CONCENTRATION;29
7.7;7. SYNTHESIS OF RADIOACTIVE COMPOUNDS;31
7.8;8. PREPARATION OF TRITIUM-LABELED COMPOUNDS;36
7.9;9. PREPARATIVE BIOSYNTHESIS;37
7.10;10. RADIATION DECOMPOSITION;39
7.11;REFERENCES;40
8;Chapter II. Units of Radioactivity and of Stable Isotopes;41
8.1;1. UNITS OF RADIOACTIVITY; PARAMETERS;41
8.2;2. SPECIFIC RADIOACTIVITY;43
8.3;3. SPECIFIC ACTIVITY PER MOLE;44
8.4;4. SPECIFIC ACTIVITY PER ATOM OF CARBON;46
8.5;5. UNITS OF STABLE ISOTOPES;47
8.6;REFERENCE;49
9;Chapter III. Health Hazards and Isotope Laboratory Design;50
9.1;1. HEALTH HAZARDS;50
9.2;2. RADIATION PROTECTION;51
9.3;3. WASTE DISPOSAL;53
9.4;4. SPILLS;53
9.5;5. THE ISOTOPE LABORATORY;54
9.6;REFERENCES;55
10;Chapter IV. Principles and Conditions for the Use of Isotopes in Biology;56
10.1;Section I. Identical Chemical and Biochemical Behavior of Isotopic Compounds;56
10.2;Section II. Constant Natural Abundance;69
10.3;Section III. Chemical Stability of Isotopic Compounds;73
10.4;REFERENCES;76
11;Chapter V. Methods;77
11.1;Section I. The Isotope Dilution Method;77
11.2;Section II. Paper Chromatography and Autoradiography;92
11.3;REFERENCES;104
12;Chapter VI. Application of the Isotope Technique;105
12.1;Section I. Gross Distribution Studies;105
12.2;Section II. Molecular Distribution Studies;114
12.3;Section III. Rates of Reactions in Living Organisms;155
12.4;REFERENCES;176
13;INDEX;178