Manual of Immunopathological Techniques

Erscheinungsjahr 2014
ISBN: 978-93-5152-084-9
Verlag: Jaypee Brothers Medical Publishers

Buch, Englisch, 180 Seiten, Format (B × H): 171 mm x 241 mm, Gewicht: 290 g

The use of immunopathological techniques has become increasingly widespread due to their ability to not only diagnose infectious and immunodeficiency disorders, but also haematological, oncological and gastroenterological diseases.

This manual is a practical guide to immunopathological techniques for undergraduates and trainees. Beginning with an overview of immunopathology, the following chapters provide in depth discussion on different techniques for the diagnosis and management of numerous immunological disorders, including autoimmune diseases, tumours, transplants and immunohistochemistry.

Presented in bullet point format for easy reference, this useful text is enhanced by high quality photographs and tables to assist learning.

Key points

- Practical guide to immunopathological techniques

- Covers different techniques for diagnosis and treatment of numerous disorders

- Presented in bullet point format for easy reference

- Highly illustrated with clinical photographs and tables

Autoren/Hrsg.

Usha

Sonkar, Gyanendra Kumar

Singh, Sangeeta

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Immunologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pathologie, Cytopathologie, Histopathologie

Inhaltsverzeichnis

- Introduction

- Tests for cell mediated immunity (CMI)

- Lymphocytes typing by Flow Cytometry

- Diagnosis of Kala-Azar by PCR technique

- Test for myeloma profile and qualitative assessment of serum proteins

- Agarose gel electrophoresis for qualitative assessment of serum proteins

- Cellulose Acetate Electrophoresis

- Agar Gel Immunoelectrophoresis for typing of myeloma

- Micro Agar Gel Double Diffusion Technique

- Counter Current Immunoelectrophoresis

- Single Radial Immunodiffusion and Immunoturbidometric methods for the estimation of Immunoglobulins

- Estimation of complement C3, C4 and hemolytic assay for serum complement

- Demonstration of immune complexes and complement by indirect immuno peroxidase method

- Test for autoimmune diseases

- Test for Autoantibodies by ELISA technique

- Acid Fuchsin Orange G in kidney biopsy for demonstration of immune complexes

- Detection of tumor markers in paraffin embedded sections by immunohistochemistry

- Detection of circulating immune complexes (CIC) in the serum

- Demonstration of immune complexes, antibodies and complements in renal biopsy by immunofluorescence

- HLA typing and Cross Match test in solid organ transplantation

- HLA B27 typing by PCR method

- Fluorescence in-situ hybridisation: methods and applications

- Detection of cytokines (IL-6, IL-8, TNF-a)

- Western Blot or Tests for detection of antibody to extractable nuclear antigen

- Detection of anti-thyroglobulin antibodies by hemagglutination method

- Distillation and demineralization of water

- Raising of polyclonal anti human anti-IgG in rabbit

Über Autor(innen)

Usha MD

Professor and Head, Department of Pathology; Incharge, UGC Advanced Immunodiagnostic Training & Research Centre, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India

Gyanendra Kumar Sonkar PhD

Assistant Professor, Department of Biochemistry, King George’s Medical University, Lucknow, Uttar Pradesh, India

Sangeeta Singh PhD

Ex-Senior Research Fellow, UGC Advanced Immunodiagnostic Training and Research Centre, Division of Immunopathology, Department of Pathology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India