Plant Stress Tolerance

Methods and Protocols
1. Auflage 2010
ISBN: 978-1-60761-701-3
Verlag: Humana Press

Buch, Englisch, Band 639, 386 Seiten, Format (B × H): 187 mm x 265 mm, Gewicht: 2010 g

Reihe: Methods in Molecular Biology

A number of abiotic factors such as drought, salinity, extreme temperatures, low or high light intensity, and deficiency or toxic levels of nutrients have huge impacts on crop productivity, and a furthering of our understanding of the molecular, biochemical, and physiological basis of stress tolerance has been widely recognized as critical. In , expert researchers cover the most important widely-used techniques, including cutting-edge strategies, in a manner that ensures effective results. Beginning with reviews on dehydration, salinity, and cold tolerance as well as on oxidative stress, the volume then continues with methods involving topics such as describing the identification of stress-regulated genes, proteins, and microRNAs using diverse approaches, measurement of osmotic adjustment, proline levels, enzymes involved in proline metabolism, and sugars as well as determination of ROS levels, lipid peroxidation, ion leakage, and the enzymes involved in ROS detoxification. Written in the highly successful ™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, provides a wide range of easy-to-follow protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Sunkar, Ramanjulu

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Botanik Pflanzenreproduktion, Verbreitung, Genetik
• Naturwissenschaften Biowissenschaften Botanik Pflanzenphysiologie, Photosynthese
• Naturwissenschaften Biowissenschaften Botanik Phytotomie, Pflanzenmorphologie

Inhaltsverzeichnis

Reviews and Overview Chapters.- Dehydration Tolerance in Plants.- Approaches to Identifying Genes for Salinity Tolerance and the Importance of Timescale.- Gene Regulation During Cold Stress Acclimation in Plants.- Redox-Dependent Regulation, Redox Control and Oxidative Damage in Plant Cells Subjected to Abiotic Stress.- Array Platforms and Bioinformatics Tools for the Analysis of Plant Transcriptome in Response to Abiotic Stress.- Metabolic Engineering of Glyoxalase Pathway for Enhancing Stress Tolerance in Plants.- Genome-Wide Approaches for Identification of Stress Regulated Genes, Proteins, and Small RNAs.- Genetic Screens to Identify Plant Stress Genes.- Arabidopsis Tiling Array Analysis to Identify the Stress-Responsive Genes.- Identification of Stress-Responsive Genes in Plants Using Suppression Subtraction Hybridization: Ozone Stress as an Example.- Identification of DNA-Binding Proteins and Protein-Protein Interactions by Yeast One-Hybrid and Yeast Two-Hybrid Screen.- Functional Characterization of Water-Deficit Stress Responsive Genes Using RNAi.- Difference Gel Electrophoresis as a Tool to Discover Stress-Regulated Proteins.- Thiol–Disulfide Redox Proteomics in Plant Research.- Cloning of Stress-Responsive MicroRNAs and other Small RNAs from Plants.- An Array Platform for Identification of Stress-Responsive MicroRNAs in Plants.- Antioxidant Enzymes and Metabolites.- Spectrophotometric Assays for Antioxidant Enzymes in Plants.- Affinity Purification and Determination of Enzymatic Activity of Recombinantly Expressed Aldehyde Dehydrogenases.- Determination and Detection of Reactive Oxygen Species (ROS), Lipid Peroxidation, and Electrolyte Leakage in Plants.- Osmotic Adjustment and Ion Measurements.- Quantification of Water Stress-Induced Osmotic Adjustment andProline Accumulation for Arabidopsis thaliana Molecular Genetic Studies.- Methods for Determination of Proline in Plants.- A New Method for Accurately Measuring ?1-Pyrroline-5-Carboxylate Synthetase Activity.- Extraction and Analysis of Soluble Carbohydrates.- Measuring Soluble Ion Concentrations (Na+, K+, Cl?) in Salt-Treated Plants.