Su Pancreatic Cancer

Methods and Protocols
2. Auflage 2013
ISBN: 978-1-62703-287-2
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 980, 389 Seiten, eBook

Reihe: Methods in Molecular Biology

Pancreatic ductal adenocarcinoma is the fifth leading cause of cancer death in the USA.  Pancreatic cancer develops as a result of the accumulation of genetic and epigenetic alterations in cancer-causing genes such as oncogenes and tumor-suppressor genes.  The second edition of Pancreatic Cancer: Methods and Protocols provides a broad range of protocols for molecular, cellular, pathological, and statistical analyses of sporadic and familial pancreatic cancer.   It covers topics from in-vitro cell cultures to in-vivo mouse models, DNA to protein manipulation, and genetic and epigenetic analyses to treatment development. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, written by highly renowned investigators with expertise in pancreatic cancer, Pancreatic Cancer: Methods and Protocols, Second Edition is an invaluable source of proven protocols to those who are interested in joining the fight against pancreatic cancer.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Su, Gloria H

Inhaltsverzeichnis

Identification and Analysis of Precursors to Invasive Pancreatic Cancer.- Tissue Microarrays: Construction and Use.- Immunohistochemistry of Pancreatic Neoplasia.- A Method for Conducting Highly Sensitive microRNA in situ Hybridization and Immunohistochemical Analysis in Pancreatic Cancer.- Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microdissection Methods for Biomedical Applications.- Considerations for Sequencing Analyses of Pancreatic Cancer Progression and Metastasis.- DNA Methylation Analysis in Human Cancer.- Quantitative Real-Time PCR Expression Analysis of Peripheral Blood Mononuclear Cells in Pancreatic Cancer Patients.- Development of a Cytokine-modified Allogeneic Whole Cell Pancreatic Cancer Vaccine.- The Prevention and Genetics of Pancreatic Cancer – A Programmatic Approach.- Development of Orthotopic Pancreatic Tumor Mouse Models.- Evaluating Dietary Compounds in Pancreatic Cancer Modeling Systems.- Quantification of Murine Pancreatic Tumors by High Resolution Ultrasound.- Microdissection and Culture of Murine Pancreatic Ductal Epithelial Cells.- Sphere-forming Assays for Assessment of Benign and Malignant Pancreatic Stem Cells.- Analysis of Transplanted Bone Marrow Derived Cells in Chronic Pancreatitis.- Analysis of the Potential for Pancreatic Cancer Metastasis In Vitro and In Vivo.- The use of Fluorescent Probes in the Study of Reactive Oxygen Species in Pancreatic Cancer Cells.- Analysis of Tumor Associated Mucin Glycotopes by Western Transfer Methods.- Evaluating Tumor Angiogenesis.- Pooled Genome-wide RNAi Screenings.- Pooled ShRNA screenings: Computational Analysis.