Roberts / Mullany | Clostridium difficile | Buch | 978-1-4939-5701-9 | sack.de

Buch, Englisch, Band 646, 241 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 482 g

Reihe: Methods in Molecular Biology

Roberts / Mullany

Clostridium difficile

Methods and Protocols
Softcover Nachdruck of the original 1. Auflage 2010
ISBN: 978-1-4939-5701-9
Verlag: Humana Press

Methods and Protocols

Buch, Englisch, Band 646, 241 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 482 g

Reihe: Methods in Molecular Biology

ISBN: 978-1-4939-5701-9
Verlag: Humana Press


, a major nosocomial pathogen shown to be a primary cause of antibiotic-associated disease, has emerged as a highly transmissible and frequently antibiotic-resistant organism, causing a considerable burden on health care systems worldwide. In , expert researchers bring together the most recently developed methods for studying the organism, including techniques involving isolation, molecular typing, genomics, genetic manipulation, and the use of animal models.  Written in the highly successful ™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, serves as an ideal guide for scientists now in a position to gain an in-depth understanding of how this organism is transmitted and how it causes disease.

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Professional/practitioner

Weitere Infos & Material


to Clostridium difficile and the Disease It Causes.- Clostridium difficile: No Longer an Enigmatic Pathogen?.- Clostridium difficile and the Disease It Causes.- Isolation and Culture Techniques.- Clostridium difficile Isolation and Culture Techniques.- Methods for Typing and Epidemiological Studies.- Molecular Typing Methods for Clostridium difficile: Pulsed-Field Gel Electrophoresis and PCR Ribotyping.- Clostridium difficile Toxinotyping.- Multilocus Sequence Typing for Clostridium difficile.- Biochemistry of the Organism.- Molecular Methods to Study Transcriptional Regulation of Clostridium difficile Toxin Genes.- Dissecting the Cell Surface.- Human Intestinal Epithelial Response(s) to Clostridium difficile.- Genomics.- Comparative Genome Analysis of Clostridium difficile Using DNA Microarrays.- Development of Systems for Genetic Analysis of the Organism.- ClosTron-Targeted Mutagenesis.- Methods for Gene Cloning and Targeted Mutagenesis.- Transposon Mutagenesis in Clostridium difficile.- Animal Models of Disease.- Refinement of the Hamster Model of Clostridium difficile Disease.- Methods for Working with the Mouse Model.



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