Overview
- A comprehensive up-to-date account of the methodologies and protocols for conventional and modern molecular techniques, currently in use for studying the gut microbial ecology of ruminants
- Written by an expert group of scientists working in the area of ruminant digestion and gut microbiology
- Written in a recipe-like format designed for direct practical use in the laboratory
- Only book in the market presenting recent molecular methodologies for studying gut microbial ecology in ruminants
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About this book
Asaresultofvarioushumanactivities,suchasincreaseinhumanpopulation,decrease in arable land due to soil degradation, urbanization, industrialization and associated increase in the demand for livestock products, dramatic changes are occurring in the global ruminant livestock sector. These changes includeshift inthesize of regional livestock populations and in the types of management and feeding systems under which ruminant livestock are held, and increased demand of a wider range of quality attributes from animal agriculture, not just of the products themselves but also of the methods used in their production. The livestock sector will need to respond to newchallengesofincreasinglivestockproductivitywhileprotectingenvironmentand human health and conservingbiodiversity and natural resources. The micro-organisms in the digestive tracts of ruminant livestock have a profound in?uence on the conversion offeedinto end products, which can impact on the- imal and theenvironment. As the livestock sector grows particularly in developing countries, there will be an increasing need to understand these processes for b- ter management and use ofbothfeed and other natural resources that underpinthe development of sustainable feeding systems.
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Keywords
Table of contents (17 chapters)
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Front Matter
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Designing **in vivo microbial ecology studies
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Classical methods for isolation, enumeration, cultivation and functional assays of rumen microbes
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PCR-based methods for analysis of populations and gene expression
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Molecular fingerprinting techniques for genotypic analysis of pure cultures and microbial communities
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DNA clone libraries of microbial communities
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Use of small subunit ribosomal RNA directed oligonucleotide probes for microbial population studies
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Genomic analysis of microbial ecosystems
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Back Matter
Editors and Affiliations
Bibliographic Information
Book Title: Methods in Gut Microbial Ecology for Ruminants
Editors: Harinder P.S. Makkar, Christopher S. McSweeney
DOI: https://doi.org/10.1007/1-4020-3791-0
Publisher: Springer Dordrecht
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: Springer Science+Business Media B.V. 2005
Hardcover ISBN: 978-1-4020-3790-0Published: 03 November 2005
Softcover ISBN: 978-90-481-6966-5Published: 10 November 2010
eBook ISBN: 978-1-4020-3791-7Published: 23 February 2006
Edition Number: 1
Number of Pages: XII, 226
Topics: Zoology, Agriculture, Ecology, Microbial Ecology, Molecular Ecology, Microbiology