Pico de Coaña Immune Checkpoint Blockade

Methods and Protocols
Erscheinungsjahr 2019
ISBN: 978-1-4939-8979-9
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1913, 225 Seiten, eBook

Reihe: Methods in Molecular Biology

This detailed volume describes a series of techniques that are essential for evaluating the efficacy of new checkpoint blockade therapies as well as understanding the mechanisms behind the therapies that have already been approved. Beginning with a section on describing the tumor microenvironment and evaluating the immune system at a systemic level, the book continues by covering functional assays that provide answers to questions that may be raised after studying the immune system and its responses to immunotherapies, as well as the use of animal models in this research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Immune Checkpoint Blockade: Methods and Protocols serves to aid researchers in furthering our understanding of checkpoint blockage as well as the study of tumor immunology and the development of new immunotherapies.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Pico de Coaña, Yago

Inhaltsverzeichnis

Double Immunohistochemistry and Digital Image Analysis.- Multispectral Fluorescence Imaging Allows for Distinctive Topographic Assessment and Sub-Classification of Tumor Infiltrating and Surrounding Immune Cells.- Systems Level Immune Monitoring by Mass Cytometry.- Immune Monitoring of Cancer Patients by Multi-Color Flow Cytometry.- High-Throughput, Fast and Sensitive Immunopeptidomics Sample Processing for Mass Spectrometry.- Identification of Immune Modulatory miRNAs by miRNA Enrichment via RNA Affinity Purification.- Expansion of Tumor-Infiltrating Lymphocytes from Melanoma Tumors.- Establishment of Bi-Dimensional (2D) and Three-Dimensional (3D) Melanoma Primary Cultures as a Tool for In Vitro Drug Resistance Studies.- Assessment of Anti-Tumor T Cell Responses by Flow Cytometry after Co-Culture of Tumor Cells with Autologous Tumor-Infiltrating Lymphocytes.- Expansion and Determination of Antigen-Reactive T Cells by Flow Cytometry.- Assessing Canonical and Adaptive Natural Killer Cell Function in Suppression Assays In Vitro.- Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Chromium Release Assay.- Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Flow Cytometry.- Evaluation of Breast Cancer and Melanoma Metastasis in Syngeneic Mouse Models.- Establishment of Melanoma Tumor Xenograft Using Single Cell Line Suspension and Co-Injection of Patient-Derived T Cells in Immune Deficient NSG Mice.- Analysis of Tissue Resident Immune Cells from Mouse Skin and Lungs by Flow Cytometry.