Calcium Measurement Methods

2010
ISBN: 978-1-61779-677-7
Verlag: Humana Press

Buch, Englisch, Band 43, 256 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 511 g

Reihe: Neuromethods

Since the introduction of fluorescent calcium indicators and the subsequent development of capacities for real-time monitoring and imaging of calcium movements in the intact cells studied in isolation, in situ and in vivo, the complex and vital calcium signaling system has been illuminated, proving calcium signals to be excellent universal reporters of cellular activity. In "Calcium Measurement Methods", a team of renowned experts in the field contribute methods dedicated to calcium imaging in neural cells with a balance between the main principles of calcium imaging and specific applications of the technique to neural tissues. The book's contents show how the combination of different indicators and recently developed microscopic techniques lead to spectacular successes in the imaging of neural cells in the living brain in both physiological and pathophysiological models. As a volume in the successful Neuromethods™ series, the chapters provide authoritative reviews including up-to-date detailed protocols of the most commonly used approaches in the field.

Cutting-edge and concise, "Calcium Measurement Methods" serves as an ideal reference to aid the many scientists worldwide engaged in calcium imaging of the nervous system.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Petersen, Ole H.

Alexei, Verkhratsky

Verkhratsky, Alexej

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Neurologie, Klinische Neurowissenschaft
• Interdisziplinäres Wissenschaften Wissenschaften Interdisziplinär Neurowissenschaften, Kognitionswissenschaft
• Naturwissenschaften Biowissenschaften Biowissenschaften Neurobiologie, Verhaltensbiologie

Inhaltsverzeichnis

1. Principles of the Ca2+ Homeostatic/Signalling System

Alexei Verkhratsky and Ole H. Petersen

2. Ca2+ Recordings: Hardware and Software (From Microscopes to Cameras)

Emil C. Toescu and Jeremy Graham

3. Ca2+ Imaging: Principles of Analysis and Enhancement

Fabio Mammano and Mario Bortolozzi

4. Bioluminescent Ca2+ Indicators

Laura Fedrizzi and Marisa Brini

5. Monitoring Calcium Levels With Genetically Encoded Indicators

Olga Garaschuk and Oliver Griesbeck

6. Intracellular Calcium-Sensitive Microelectrodes

Roger C. Thomas

7. Ca2+ Caging and Uncaging

Shin Hye Kim and Myoung Kyu Park

8. Ca2+ Imaging of Intracellular Organelles: Endoplasmic Reticulum

Robert Blum, Ole H. Petersen, and Alexei Verkhratsky

9. Ca2+ Imaging of Intracellular Organelles: Mitochondria

Lucía Núñez, Carlos Villalobos, María Teresa Alonso, and Javier García-Sancho

10. Ca2+ Imaging of Dendrites and Spines

Knut Holthoff

11. In vivo Ca2+ Imaging of the Living Brain Using Multi-Cell Bolus Loading Technique

Gerhard Eichhoff, Yury Kovalchuk, Zsuzsanna Varga, Alexei Verkhratsky, and Olga Garaschuk

12. Ca2+ Imaging of Glia

Christian Lohr and Joachim W. Deitmer