Handbook of Industrial Cell Culture

Mammalian, Microbial, and Plant Cells
2003
ISBN: 978-1-58829-032-8
Verlag: Humana Press

Buch, Englisch, 536 Seiten, Format (B × H): 183 mm x 260 mm, Gewicht: 1348 g

A diverse team of researchers, technologists, and engineers describe, in simple and practical language, the major current and evolving technologies for improving the biocatalytic capabilities of mammalian, microbial, and plant cells. The authors present state-of-the-art techniques, proven methods, and strategies for industrial screening, cultivation, and scale-up of these cells, and describe their biotech and industrial uses. Special emphasis is given to the solving critical issues encountered during the discovery of new drugs, process development, and the manufacture of new and existing compounds. Other topics include recombinant protein expression, bioinformatics, high throughput screening, analytical tools in biotechnology, DNA shuffling, and genomics discovery.

Zielgruppe

Research

Autoren/Hrsg.

Parekh, Sarad R.

Vinci, Victor A.

Fachgebiete

• Naturwissenschaften Chemie Chemie Allgemein Chemometrik, Chemoinformatik
• Naturwissenschaften Chemie Chemie Allgemein Toxikologie, Gefahrstoffe, Sicherheit in der Chemie
• Naturwissenschaften Chemie Chemie Allgemein Chemische Labormethoden, Stöchiometrie
• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Chemie Chemie Allgemein Pharmazeutische Chemie, Medizinische Chemie
• Interdisziplinäres Wissenschaften Wissenschaften: Forschung und Information Forschungsmethodik, Wissenschaftliche Ausstattung
• Technische Wissenschaften Verfahrenstechnik | Chemieingenieurwesen | Biotechnologie Biotechnologie Industrielle Biotechnologie

Inhaltsverzeichnis

I. Mammalian Cell Culture.- 1 Analysis and Manipulation of Recombinant Glycoproteins Manufactured in Mammalian Cell Culture.- 2 • Genetic Approaches to Recombinant Protein Production in Mammalian Cells.- 3 • Protein Expression Using Transgenic Animals.- 4 • Mammalian Cell Culture: Process Development Considerations.- II. Microbial Cell Culture.- 5 • Natural Products: Discovery and Screening.- 6 • Genetic Engineering Solutions for Natural Products in Actinomycetes.- 7 • Culture Medium Optimization and Scale-Up for Microbial Fermentations.- III. Plant Cell Culture.- 8 • Functional Genomics for Plant Trait Discovery.- 9 • Molecular Tools for Engineering Plant Cells.- 10 • Plant Cell Culture: A Critical Tool for Agricultural Biotechnology.- 11 • Expression of Recombinant Proteins via the Plastid Genome.- 12 • Oleosin Partitioning Technology for Production of Recombinant Proteins in Oil Seeds.- IV. Critical Tools for Biotechnology.- 13 • Genome Sequencing and Genomic Technologies in Drug Discovery and Development.- 14 • Proteomics.- 15 • Metabolic Flux Analysis, Modeling, and Engineering Solutions.- 16 • Advances in Analytical Chemistry for Biotechnology: Mass Spectrometry of Peptides, Proteins, and Glycoproteins.- 17 • DNA Shuffling for Whole Cell Engineering.- 18 • Cell Culture Preservation and Storage for Industrial Bioprocesses.