Clinical Applications of Capillary Electrophoresis

1999. Auflage 1999
ISBN: 978-0-89603-639-0
Verlag: Humana Press

Buch, Englisch, Band 27, 212 Seiten, Format (B × H): 155 mm x 234 mm, Gewicht: 1100 g

Reihe: Methods in Molecular Medicine

The term “electrophoresis” was first used by Michaelis in 1909, to - scribe the migration of colloids in an electric field. The first practical elect- phoresis method was described by Tiselius in 1937. He used a U-tube filled with buffer layered on top of sample; migration could be monitored using Schlieren optics. In zone electrophoresis, the U-tube was replaced by paper, a support material employed simply to prevent or minimize diffusion of ions, so that ions applied in a narrow strip to the paper will separate and remain as relatively discrete zones. Paper was superceded by a variety of other media, - cluding cellulose acetate, hydrolyzed starch (starch gel), agarose, and polyacry- mide. The latter, in addition to being a support medium, has size-sieving properties. From the basic zone electrophoresis, other means of separation have been dev- oped. These include, isoelectric focusing, isotachophoresis, density gradient el- trophoresis, and various forms of immunoelectrophoresis. In some ways Capillary Electrophoresis (CE) has gone full circle back to the original method of Tiselius. In its simplest form, separations occur in a buffer solution within a glass (fused silica) tube and detection occurs as sample moves past an optical window. CE has rapidly developed into a technique that rivals HPLC in its versatility. All the classical electrophoretic separations—zone, IEF, and isotachophoresis—have their counterparts in CE. Excitingly so, and - thoritatively treated in Clinical Applications of Capillary Electrophoresis.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Palfrey, Stephen M

Fachgebiete

• Technische Wissenschaften Sonstige Technologien | Angewandte Technik Medizintechnik, Biomedizintechnik
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Innere Medizin
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizin, Gesundheitswesen Medizintechnik, Biomedizintechnik, Medizinische Werkstoffe
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pathologie, Cytopathologie, Histopathologie

Inhaltsverzeichnis

Clinical Applications of Capillary Electrophoresis.- Serum Protein Electrophoresis.- Urine Proteins.- Electrophoresis of Cerebrospinal Fluid.- Immunosubtraction as a Means of Typing Monoclonal and Other Proteins in Serum and Urine.- Analysis and Classification of Serum Cryoglobulins.- Myoglobin Analysis.- Enzyme Analysis.- Quantification of Human Cytomegalovirus by Competitive PCR and Capillary Electrophoresis.- Laboratory Diagnosis of Structural Hemoglobinopathies and Thalassemias by Capillary Isoelectric Focusing.- Serum Apolipoproteins.- Gene Dosage in Capillary Electrophoresis.- Rapid Analysis of Amplified Double-Stranded DNA by Capillary Electrophoresis with Laser-Induced Fluorescence Detection.- Identification of Mutated p53 in Cancers by Nongel-Sieving Capillary Electrophoretic SSCP Analysis.- Detection of Microsatellite Instability in Cancers by Means of Nongel-Sieving Capillary Electrophoresis.- Serum Lamotrigine Analysis.- Acetonitrile Stacking.- Confirmation of the Presence of Drugs of Abuse in Urine.- Steroid Analysis by Micellar Electrokinetic Capillary Chromatography.- Determination of Polyamines by Capillary Electrophoresis.- Urinary Oxalate and Citrate.- Plasma Nitrite and Nitrate Determination.