Liver Stem Cells

Methods and Protocols
Softcover Nachdruck of the original 1. Auflage 2012
ISBN: 978-1-4939-5910-5
Verlag: Humana Press

Buch, Englisch, Band 826, 226 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 453 g

Reihe: Methods in Molecular Biology

Increasing evidence suggests that liver stem cells have the capacity to differentiate into parenchymal hepatocytes or into bile ductular cells. These stem cells may be activated to proliferate after severe liver injury or exposure to hepatocarcinogens. Stem cell replacement strategies are being investigated as an alternative approach to liver repair and regeneration. Additionally, stem cell transplantation has been shown to significantly improve liver function and increase survival in experimentally-induced liver-injury models in animals. In Liver Stem Cells: Methods and Protocols, expert researchers focus on  several hepatic progenitor cells, hepatic differentiation form stem cells, bile ductal cell formation from stem cells, liver stem cells and hepatocarcinogenesi, and  application of liver stem cells for cell therapy.  These topics shed light on stem cell technology which may lead to the development of effective clinical modalities for human liver diseases. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Thorough and intuitive, Liver Stem Cells: Methods and Protocols seeks to aid scientists in the further study of preclinical and clinical investigations that explore the therapeutic potential of stem cells in repair of liver injuries.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Ochiya, Takahiro

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Hepatologie
• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

Purification and Culture of Fetal Mouse Hepatoblasts that are Precursors of Mature Hepatocytes and Biliary Epithelial Cells.-Clinical Uses of Liver Stem Cells.-Identification and Isolation of Adult Liver Stem/Progenitor Cells.-Isolation and Purification Method of Mouse Fetal Hepatoblasts.-Isolation of Hepatic Progenitor Cells from the Galactosamine-Treated Rat Liver.-Purification of Adipose Tissue Mesenchymal Stem cells (AT-MSC) and Differentiation Towards Hepatic-Like Cells.-Development of Immortalized Hepatocyte-like Cell from hMSC.-Isolation of Adult Human Pluripotent Stem Cells from Mesenchymal Cell Populations and their Application to Liver Damages.-Generation and Hepatic Differentiation of Human iPS Cells.-Efficient Hepatic Differentiation from Human iPS cells by Gene Transfer.-“Tet-On” System Toward Hepatic Differentiation of Human Mesenchymal Stem Cells by Hepatocyte Nuclear Factor.-SAMe and HuR in Liver Physiology Usefulness of Stem Cells in Hepatic Differentiation Research.-Transdifferentiation of Mature Hepatocytes into Bile Duct Cells within a Collagen Gel Matrix.-Identification of Cancer Stem Cell-Related MicroRNAs in Hepatocellular Carcinoma.-Intravenous Human Mesenchymal Stem Cells (MSC) Transplantation in NOD/SCID Mice Preserve Liver Integrity of Irradiation Damage.-Engineering of Implantable Liver Tissues.-Mesenchymal Stem Cell Therapy on Murine Model of Non-Alcoholic Steatohepatitis.