Protocols for In Vitro Propagation of Ornamental Plants

2010
ISBN: 978-1-61779-673-9
Verlag: Humana Press

Buch, Englisch, Band 589, 400 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 779 g

Reihe: Methods in Molecular Biology

It was around 1970, I had just completed a 5-year breeding project aiming at fxing fower colour in gerbera progenies: white, yellow, pink, and red; colour homogeneity was sound, but size and shape still required some improvement. The problem was defnitely resolved by Murashige and Skoog, USA who published a reliable protocol for gerbera micropro- gation. In short, my gerbera seed lines were immediately rendered obsolete by this e- cient cloning system, able to produce millions of plants of a matchless and previously unknown homogeneity, the uniformity of fower shape and colour being the basic requi- ments for the market. The success of micropropagation resulted in a tremendous growth in gerbera fower production worldwide, and this species conquered a leading place in the foriculture industry. This personal experience stresses the impact of micropropagation on the genetic improvement research strategies in ornamentals. Micropropagation has become “in- sive”, especially in ornamental plant material issues. Today, hundreds of protocols exist; however, only a modest percentage of them are exploited economically. Thus, only micropropagation of plants with a high market price range, like orchids for instance, has proved cost-effective and achieved great success. Micropropagation is a labour-intensive system: hand-power is estimated to rep- sent 60–70% of total costs. This explains the outsourcing of the major labs in developing countries where labour is cheaper. Nevertheless, certain industrial protocols remain a proprietary technology of leading labs, mostly western, with the exception of Japan and Taiwan.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Ochatt, Sergio J.

Jain, Shri Mohan

Fachgebiete

• Naturwissenschaften Biowissenschaften Botanik

Inhaltsverzeichnis

Protocols for In Vitro Propa gation of Ornamental Plants.- Micropropagation and Organogenesis of Anthurium andreanum Lind cv Rubrun.- A Highly Efficient Protocol for Micropropagation of Begonia tuberous.- Micropropagation of Vanda teres (Roxb.) Lindle.- In vitro Propagation Through Axillary Shoot Culture of Ranunculus asiaticus L.- Micropropagation of Crataeva adansonii D.C. Prodr: An Ornamental Avenue Tree.- Strategies for the Micropropagation of Bromeliads.- Micropropagation of Poinsettia by Organogenesis.- Micropropagation of Phalaenopsis Blume.- Genetic Transformation of Carnation (Dianthus caryophylus L.).- Matrix Supported Liquid Culture and Machine Vision Analysis of Regenerated Shoots of Gladiolus.- In Vitro Propagation of Carnation (Dianthus caryophyllus L.).- In Vitro Propagation of Jasminum officinale L.: A Woody Ornamental Vine Yielding Aromatic Oil from Flowers.- Micropropagation of Lysionotus pauciflorus Maxim. (Gesneriaceae).- Micropropagation of Rhododendron.- In Vitro Regeneration and Multiplication of Passiflora Hybrid “Guglielmo Betto”.- In Vitro Propagation of Rose.- In Vitro Propagation of Chrysanthemum.- Micropropagation of Codiaeum variegatum (L.) Blume and Regeneration Induction via Adventitious Buds and Somatic Embryogenesis.- Methods for In Vitro Propagation of Pelargonium x Hortorum and Others: From Meristems to Protoplasts.- Marigold Regeneration and Molecular Analysis of Carotenogenic Genes.- Somatic Embryogenesis in In Vitro Culture of Leucojum vernum L.- In Vitro Propagation of Australian Native Ornamental Plant, Scaevola.- Micropropagation of Tulip: Production of Virus-Free Stock Plants.- In Vitro Propagation of Ornamental Myrtus (Myrtus communis).- Sunflower Propagation.- Clonal Propagation of Cyclamen persicum Via SomaticEmbryogenesis.- Section B Protocols for In Vitro Propa gation of Ornamental Plants.- In Vitro Production of Sweet Peas (Lathyrus odoratus L.) via Axillary Shoots.- In Vitro Conservation and Cryopreservation of Ornamental Plants.- Genetic Engineering of Novel Flower Colors in Floricultural Plants: Recent Advances via Transgenic Approaches.- Azalea Phylogeny Reconstructed by Means of Molecular Techniques.- Status of Floriculture in Europe.- Thin Cell Layers: Power-Tool for Organogenesis of Floricultural Crops.