Müller / Masquida / Winkler Ribozymes

Principles, Methods, Applications
1. Auflage 2021
ISBN: 978-3-527-81453-4
Verlag: Wiley-VCH
Format: EPUB
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 944 Seiten, E-Book

Timely and unique in its breadth of content, this all-encompassing and authoritative reference on ribozymes documents the great diversity of nucleic acid-based catalysis. It integrates the knowledge gained over the past 35 years in the field and features contributions from virtually every leading expert on the subject.

 

'Ribozymes' is organized into six major parts. It starts by describing general principles and strategies of nucleic acid catalysis. It then introduces naturally occurring ribozymes and includes the search for new catalytic motifs or novel genomic locations of known motifs. Next, it covers the development and design of engineered ribozymes, before moving on to DNAzymes as a close relative of ribozymes. The next part examines the use of ribozymes for medicinal and environmental diagnostics, as well as for therapeutic tools. It finishes with a look at the tools and methods in ribozyme research, including the techniques and assays for structural and functional characterization of nucleic acid catalysts.

 

The first reference to tie together all aspects of the multi-faceted field of ribozymes

* Features more than 30 comprehensive chapters in two volumes

* Covers the chemical principles of RNA catalysis; naturally occurring ribozymes, engineered ribozymes; DNAzymes; ribozymes as tools in diagnostics and therapy, and tools and methods to study ribozymes

* Includes first-hand accounts of concepts, techniques, and applications by a team of top international experts from leading academic institutions

* Dedicates half of its content to methods and practical applications, ranging from bioanalytical tools to medical diagnostics to therapeutics

 

Ribozymes is an unmatched resource for all biochemists, biotechnologists, molecular biologists, and bioengineers interested in the topic.

Autoren/Hrsg.

Müller, Sabine

Masquida, Benoît

Winkler, Wade

Inhaltsverzeichnis

INTRODUCTION

The chemical principles of RNA catalysis

 

NATURALLY OCCURRING RIBOZYMES

Biological roles of self-cleaving ribozymes

Chemical mechanisms of the nucleolytic ribozymes

The glmS ribozyme and its multifunctional coenzyme glucosamine-6-phosphate

The lariat capping ribozyme

Self-splicing group II introns

The Spliceosome: an RNA-Protein ribozyme derived from ancient mobile genetic elements

The ribosome and protein synthesis

The RNase P ribozyme

Ribozyme Discovery in Bacteria

Small self-cleaving ribozymes in the genomes of vertebrates

 

ENGINEERED RIBOZYMES

Phosphoryl transfer ribozymes

RNA replication and the RNA polymerase ribozyme

Maintenance of genetic information in the first ribocell

Ribozyme-catalyzed RNA recombination

Engineering of hairpin ribozymes for RNA processing reactions

Engineering of the Neurospora Varkud Satellite ribozyme for cleavage of non-natural stem-loop substrates

Chemical modifications in natural and engineered ribozymes

Ribozymes for regulation of gene expression

Development of flexizyme aminoacylation ribozymes and their applications

In vitro selected (deoxy)ribozymes that catalyze carbon-carbon-bond formation

Nucleic acid catalyzed RNA ligation and labeling

 

DNAZYMES

The chemical repertoire of DNA enzymes

Light-utilizing DNAzymes

Applications of DNAzymes in computing and nanotechnology.

 

RIBOZYMES AS TOOLS IN DIAGNOSTICS AND THERAPY

Optimization of antiviral ribozymes

DNAzymes as Biosensors

Compartmentalization-based technologies for in vitro selection and evolution of ribozymes and light-up RNA aptamers

 

TOOLS AND METHODS TO STUDY RIBOZYMES

Elucidation of ribozyme mechanisms at the example of the Pistol ribozyme

Strategies for crystallization of natural ribozymes

NMR spectroscopic investigation of ribozymes

Studying Ribozymes with Electron Paramagnetic Resonance Spectroscopy

Computational modeling methods for 3D structure prediction of ribozymes

Über Autor(innen)

Sabine Müller is Full Professor for Biochemistry/Bioorganic Chemistry at Greifswald University (Germany). Having obtained her academic degrees from Humboldt University Berlin, she spent one and a half years as a postdoc at the MRC in Cambridge (UK) and then took up a group leader position again at Humboldt University. After finishing her Habilitation in 2003, she moved to the Ruhr University Bochum, where she was a Professor for Bioorganic Chemistry, before taking up her present appointment at Greifswald in 2006. Professor Müller has received several scientific awards, including a Heisenbergstipendium of the Deutsche Forschungsgemeinschaft (DFG). She is a member of the Leibniz-Societät der Wissenschaften zu Berlin and of AcademiaNet.

 

Benoît Masquida is a research director at Centre National de la Recherche Scientifique. After graduating from Université Louis Pasteur in Strasbourg (France) working on the rules governing RNA folding, he spent one year at Yale University concentrating on ribosomal RNA structural motifs. After coming back to Strasbourg, he was appointed as a CNRS Research Scientist. He was promoted to Research Director in 2016, acknowledging his important contributions in the RNA structural biology field.

 

Wade Winkler is an Associate Professor of Cell Biology and Molecular Genetics at the University of Maryland (USA). He acquired a graduate degree from The Ohio State University before spending three years as a postdoctoral researcher at Yale University. In 2004, he began his independent scientific career at The University of Texas Southwestern Medical Center in the Department of Biochemistry, and relocated his laboratory to the University of Maryland in 2011. Through the years, Professor Winkler has authored multiple influential publications on the different types of regulatory RNAs in bacteria.