Receptor Purification

Receptors for Steroid Hormones, Thyroid Hormones, Water-Balancing Hormones, and Others
1991. Auflage 1990
ISBN: 978-0-89603-183-8
Verlag: Humana Press

Buch, Englisch, Band 2, 417 Seiten, Format (B × H): 183 mm x 259 mm, Gewicht: 1134 g

Reihe: Receptor Purification

Receptor Purification is a unique, practical two-volume guide to the purification of most receptor types, including receptors for neurotransmitters, polypeptide hormones, steroid hormones, and a variety of other ligands involved in many biological processes. Today's methods of receptor purification have evolved from years of research on the mechanisms of receptor actions, where they were needed to discover receptor properties and ultimately for use in cloning.

Receptor Purification provides a comprehensive source of techniques to enable the purification of cloned receptors, as well as a handy reference on purification of receptors from cells and tissues in a physiological context. Designed to give investigators the best possible selection of approaches to a purification problem, alternative methods are provided where applicable.

In addition, Receptor Purification features chapters on specific agents-such as covalent binding ligands-that will greatly simplify some purification processes.

Many of the leading laboratories whose work has led to the purification of a wide variety of receptors are represented in the contributions to these two volumes. In some chapters, information has been added on the applications of purified receptors, while others focus on the cloning of the receptors. The purification of receptors is group-based on functional ligands.

The first volume is devoted to the purification of receptors that bind neurotransmitters and a wide variety of polypeptide hormones. Volume 2 covers the steroid receptors and associated proteins, such as vitamin D-3 and retinoic acid receptors. Other polypeptide receptors, such as those involved in bacterial motion, are given attention as well. In short, the twin volumes of Receptor Purification constitute the most complete collection of purification procedures to date.

Receptor Purification is a valuable desk- and benchside resource for biologists, biochemists, pharmacologists, molecular biologists, endocrinologists, and indeed everyone-whether novice or expert-involved in any area of receptor research.

Zielgruppe

Research

Autoren/Hrsg.

Litwack, Gerald

Fachgebiete

• Naturwissenschaften Biowissenschaften Molekularbiologie
• Naturwissenschaften Biowissenschaften Biochemie (nichtmedizinisch)
• Naturwissenschaften Biowissenschaften Proteinforschung
• Naturwissenschaften Biowissenschaften Zellbiologie
• Naturwissenschaften Biowissenschaften Tierkunde / Zoologie Tierphysiologie
• Sozialwissenschaften Psychologie Allgemeine Psychologie Biologische Psychologie, Neuropsychologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Endokrinologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Neurologie, Klinische Neurowissenschaft

Inhaltsverzeichnis

Dexamethasone 21-Mesylate: Applications for Purification and Examination of Glucocorticoid Receptors.- Dexamethasone-Biotin Affinity Probes: Partial Purification of the Human Spleen Tumor Glucocorticoid Receptor.- Purification of the Unactivated/Nontransformed Glucocorticoid Receptor Complex from Rat Tissues.- Purification of the Glucocorticoid Receptor and Its Ligand-Binding Domains.- Glucocorticoid Receptor Purification.- Glucocorticoid Receptor Purification and Characterization Using Tissue Culture Cells.- The Glucocorticoid Receptor:Purification and Characterization.- Purification of the Rat Kidney Mineralocorticoid Receptor.- Purification of the Androgen Receptor.- Purification of the Estrogen Receptor.- Estrogen Receptor Purification.- Progesterone Receptor Purification.- Purification of the Nontransformed Avian Progesterone Receptor.- Purification and Characterization of Chicken Progesterone Receptor.- 1,25-Dihydroxyvitamin D Receptor Purification and Structural Determination.- Retinoic Acid-Binding Protein.- Receptor Purification of the Thyroid Hormone Receptor.- The Vasopressin V1 Receptor: Purification, Characterization and Analysis of VI Receptor Interaction(s) with GuanineNucleotide-Binding Proteins.- Purification of an Angiotensin II Receptor.- Purification of Prostaglandin E1/Prostacyclin Receptors of Human Blood Platelets.- The Asialoglycoprotein Receptor.- Periplasmic and Membrane Receptors of Bacterial Chemotaxis.- Index 413.