Lafond / Vaillancourt Human Embryogenesis

Methods and Protocols
Erscheinungsjahr 2009
ISBN: 978-1-60327-009-0
Verlag: Humana Press
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 550, 312 Seiten, eBook

Reihe: Methods in Molecular Biology

Despite its inherent controversy, the exploration of the human embryo can unlock many of the answers to our deepest biological questions. In Human Embryogenesis: Methods and Protocols , internationally recognized researchers contribute detailed methods to analyze various aspects of the embryogenesis process. While comprehensively covering subjects such as the molecular mechanisms of embryonic development, in vitro fertilization, cloning, and the laws and ethical considerations of working with embryos, the volume also addresses critical features of fetal and placental development as well as of uterine biology. Written in the highly successful Methods in Molecular Biology ™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and state-of-the-art, Human Embryogenesis: Methods and Protocols provides a firm foundation for the successful analysis of the embryogenesis process and an easily accessible description of the limitations and advantages of the techniques proposed, certain to aid all those who wish to further unravel the mysteries of human embryogenesis.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Lafond, Julie

Vaillancourt, Cathy

Inhaltsverzeichnis

Introduction: What is Human Embryogenesis?.- Human Embryogenesis: Overview.- Studying Embryo Implantation: From Trophoblasts to Embryo.- Differential Gene Expression in the Uterus and Blastocyst During the Reactivation of Embryo Development in a Model of Delayed Implantation.- Coculture of Decidua and Trophoblast to Study Proliferation and Invasion.- Isolation and Culture of Term Human Cytotrophoblast Cells and In Vitro Methods for Studying Human Cytotrophoblast Cells’ Calcium Uptake.- Endocrinology and Cell Signaling in Human Villous Trophoblast.- Gestation-Induced Uterine Vascular Remodeling.- Steriodogenesis and Reproductive System.- Placental and Fetal Steroidogenesis.- Current Methods in Investigating the Development of the Female Reproductive System.- A Revised and Improved Method for the Isolation of Seminiferous Tubule-Enriched Fractions that Preserves the Phosphorylated and Glycosylated Forms of Proteins.- A Novel Technical Approach for the Measurement of Individual ACAT-1 and ACAT-2 Enzymatic Activity in the Testis.- From Embryo to Fetal Development.- Genetic Dissection of Caenorhabditis elegans Embryogenesis Using RNA Interference and Flow Cytometry.- Immune System: Maturation of Myeloid Cells.- Functional Development of Human Fetal Gastrointestinal Tract.- Renal and Cardiac Na+–K+-ATPase and Aconitase in a Rat Model of Fetal Programming.- Embryotoxicity, in Vitro Fertilization, Cloning, and Ethics.- Assessment of Embryotoxicity Using Mouse Embryo Culture.- Fertilization In Vitro.- Animal Cloning by Somatic Cell Nuclear Transfer.- The Human Embryo: Ethical and Legal Aspects.