Krishan / Krishnamurthy / Totey Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration

1. Auflage 2011
ISBN: 978-1-118-14806-8
Verlag: John Wiley & Sons
Format: EPUB
Kopierschutz: Adobe DRM (»Systemvoraussetzungen)

E-Book, Englisch, 410 Seiten, E-Book

A much-needed primer on the use of laser flow cytometry for stemcell analysis
Laser flow cytometry is a powerful tool for rapid analysis ofcells for marker expression, cell cycle position, proliferation,and apoptosis. However, no resources specifically address the useof this methodology for the study of stem cells; this is especiallyimportant as stem cell analysis involves specialized methods andstaining procedures based on specific characteristics such asmarker expression, cell size, drug transport, and efflux of thestem cells.
Now, this book reviews these procedures, discusses the sciencebehind them, and provides real-world examples to illustrate theusefulness of the methods. It brings together world-class expertsin pathology, biophysics, immunology, and stem cell research, whodraw upon their extensive experience with the methods and showexamples of good data to help guide researchers in the rightdirection.
Chapter coverage includes:
* Stem cell analysis and sorting using side population
* Flow cytometry in the study of proliferation and apoptosis
* Stem cell biology and application
* Identification and isolation of very small embryonic-like stemcells from murine and human specimens
* Hematopoietic stem cells--issues in enumeration
* Human embryonic stem cells: long-term culture andcardiovascular differentiation
* Limbal stem cells and corneal regeneration
* Flow cytometric sorting of spermatogonial stem cells
* Breast cancer stem cells
* Stem cell marker expression in cells from body cavityfluids
This book is an essential resource for all graduate students,practitioners in developing countries, libraries and bookrepositories of universities and research institutions, andindividual researchers. It is also of interest to laboratoriesengaged in stem cell research and use of stem cells for tissueregeneration, and to any organization dealing in stem cell andtissue regeneration research.

Autoren/Hrsg.

Krishan, Awtar

Krishnamurthy, H.

Totey, Satish

Inhaltsverzeichnis

CONTRIBUTORS.
PREFACE.
1 Basics of Flow Cytometry (H. Krishnamurthy and L. ScottCram).
2 Practical Considerations for Flow Cytometric Sorting of StemCells (Geoffrey W. Osborne).
3 Stem Cell Analysis and Sorting Using Side Population Analysis(William Telford).
4 Flow Cytometry in the Study of Proliferation and Apoptosis(Michael G. Ormerod and Ronald M. Hamelik).
5 Flow Cytometric Analysis of Drug Transport and Efflux in StemCells (Awtar Krishan and Ronald M. Hamelik).
6 Stem Cell Biology and Application (Swapnil Totey, RajarshiPal, Murali Krishna Mamidi, Vijayendran Govindasamy, and SatishTotey).
7 Identification and Isolation of Very Small Embryonic-like StemCells from Murine and Human Specimens (Ewa K. Zuba-Surma,Dong-Myung Shin, Izabella Klich, Janina Ratajczak, Magda Kucia, andMariusz Z. Ratajczak).
8 Electronic Volume of Hematopoietic Stem Cells (SiddharthSharma and Awtar Krishan0.
9 Hematopoietic Stem Cells: Issues in Enumeration (MichaelKeeney and D. Robert Sutherland).
10 Embryonic Stem Cells: Development and Characterization(Vijay Bhaskar R. Konala, Villoo Morawala-Patell, and AparnaKhanna).
11 Human Embryonic Stem Cells: Long-Term Culture andCardiovascular Differentiation (Maneesha Inamdar).
12 Mesenchymal Stromal Cells and Their Clinical Applications(Jyoti Kode and Vivek Tanavde).
13 Circulating Adult Stem Cells of Hematopoietic Origin forVascular and Neural Regeneration (Lissy K. Krishnan).
14 Flow Cytometric Characterization of Neural ProgenitorsDerived from Human Pluripotent Stem Cells (Raj R. Rao, Sujoy K.Dhara, Shilpa Iyer, and David W. Machacek).
15 Limbal Stem Cells and Corneal Regeneration (Geeta K.Vemuganti, Murali Mohan Sagar Balla, and Shubha Tiwari).
16 Flow Cytometric Sorting of Spermatogonial Stem Cells (B.S. Srinag, J. M. Kalappurakkal, G.H. Mohan, and H.Krishnamurthy).
17 Breast Cancer Stem Cells (Devaveena Dey and AnnapoorniRangarajan).
18 Tumor Stem Cell Marker Expression in Cells from Body CavityFluids (Awtar Krishan, Deepti Sharma, and Ronald M.Hamelik).
INDEX.