Buch, Englisch, Band 207, 481 Seiten, Previously published in hardcover, Format (B × H): 155 mm x 235 mm, Gewicht: 744 g
Reihe: Methods in Molecular Biology
Methods and Protocols
Buch, Englisch, Band 207, 481 Seiten, Previously published in hardcover, Format (B × H): 155 mm x 235 mm, Gewicht: 744 g
Reihe: Methods in Molecular Biology
ISBN: 978-1-61737-250-6
Verlag: Humana Press
In Recombinant Antibodies for Cancer Therapy: Methods and Protocols, Martin Welschoff and Jürgen Krauss present a collection of carefully selected protocols for the design, construction, and characterization of novel anticancer therapeutics based on recombinant antibody technology. These readily reproducible methods include coverage of hybridoma-derived recombinant antibodies, recombinant antibody fragments from phagemid-displayed antibody repertoires, antibody fragments with additional properties, and large-scale production of recombinant antibodies for clinical applications. Concise review articles also survey the current status of recombinant antibodies in cancer therapy, as well as the generation of antibody molecules through antibody engineering.
Zielgruppe
Research
Autoren/Hrsg.
Fachgebiete
Weitere Infos & Material
Part I. Introduction
Generation of Antibody Molecules Through Antibody Engineering
Sergey M. Kipriyanov
Application of Recombinant Antibodies in Cancer Patients
Jürgen Krauss, Michaela Arndt, and Michael Pfreundschuh
Part II. Hybridoma-Derived Antibodies
DNA Immunization as a Means to Generate Antibodies to Proteins
Partha S. Chowdhury
Chimerization of a Monoclonal Antibody for Treating Hodgkin's Lymphoma
Jürgen Krauss, Hans Heinrich Förster, Barbara Uchanska-Ziegler, and Andreas Ziegler
Humanization of Monoclonal Antibodies by CDR Grafting
Siobhan O'Brien and Tarran Jones
Part III. Recombinant Antibody Fragments from Phagemid-Displayed Antibody Repertoires
Generation and Screening of a Modular Human scFv Expression Library from Mulitple Donors
Martin Welschof, Christiane Christ, Ingrid Hermes, Armin Keller, Christian Kleist, and Michael Braunagel
Construction of Semisynthetic Antibody Libraries
Michael Braunagel
Single-Domain VH Antibody Fragments from a Phage Display Library
Liat Binyamin, Daniel Plaksin, and Yoram Reiter
Isolation of Human Fab Fragments Against Ovarian Carcinoma Using Guided Selection
Mariangela Figini, Andrew Green, Francesco Colotta, and Silvana Canevari
Human Recombinant Fab Antibodies with T-Cell Receptor-Like Specificities Generated from Phage Display Libraries
Jan Engberg, Ali F. Yenidunya, Rikke Clausen, Liselotte B. Jensen, Peter Sørensen, Pernille Kops, and Erik Riise
Engineering Hot Spots for Affinity Enhancement of Antibodies
Partha S. Chowdhury
Simultaneous Humanization and Affinity Optimization of Monoclonal Antibodies
Herren Wu
Tailoring Kinetics of Antibodies Using Focused Combinatorial Libraries
Herren Wu and Ling-Ling An
Part IV. Antibody Fragments with Additional Properties
Engineering scFvs for Improved Stability
Partha S. Chowdhury and George Vasmatzis
Recombinant Single-Chain and Disulfide-Stabilized Fv Immunotoxins for Cancer Therapy
Revital Niv, Dina Segal, and Yoram Reiter
Generation of Recombinant Immunotoxins for Specific Targeting of Tumor-Related Peptides Presented by MHC Molecules
Cyril J. Cohen, Galit Denkberg, Dina Segal, and Yoram Reiter
Construction and Characterization of RNase-Based Targeted Therapeutics
Dianne L. Newton, Junichiro Futami, Dale Ruby, and Susanna M. Rybak
Bispecific Diabodies for Cancer Therapy
Michaela Arndt and Jürgen Krauss
Generation and Characterization of Bispecific Tandem Diabodies for Tumor Therapy
Sergey M. Kipriyanov
Generation of Recombinant Multimeric Antibody Fragments for Tumor Diagnosis and Therapy
Barbara E. Power, Alexander A. Kortt, and Peter J. Hudson
Construction and Characterization of Minibodies for Imaging and Therapy of Colorectal Carcinomas
Paul J. Yazaki and Anna M. Wu
Generation, Expression, and Monitoring of Recombinant Immune Receptors for Use in Cellular Immunotherapy
Andreas Hombach, Claudia Heuser, and Hinrich Abken
Tailoring Natural Effector Functions: Antibody Engineering Beyond Humanization
Ole H. Brekke and John E. Thommesen
Single-Chain Fv-Based Affinity Purification of the Cellular Stress Protein gp96 for Vaccine Development
Christian Kleist, Daniele Arnold-Schild, Martin Welschof, Martina Finger, Gerhard Opelz, Hans-Georg Rammensee, Hansjörg Schild, and Peter Terness
Recombinant Adenoviruses for In Vivo Expression of Antibody Fragments
Roland E. Kontermann, Tina Korn, and Valérie Jérôme
Part V. Large Scale Production of Recombinant Antibodies for Clinical Application
Production of Antibody Fragments in a Bioreactor
Halldis Hellebust.
Large Scale Production of Recombinant Antibodies by Utilizing Cellulose-Binding Domains
Itai Benhar and Yevgeny Berdichevsky
Production of Tumor-Specific Antibodies in Tobacco
Carmen Vaquero-Martin and Rainer Fischer
Index
