Hoffman Methionine Dependence of Cancer and Aging

Methods and Protocols
Erscheinungsjahr 2019
ISBN: 978-1-4939-8796-2
Verlag: Springer US
Format: PDF
Kopierschutz: 1 - PDF Watermark

E-Book, Englisch, Band 1866, 329 Seiten, eBook

Reihe: Methods in Molecular Biology

This book explores the methionine dependence of cancer and its effects on aging, a great story of science that is not widely known. The chapters in this book describe the discovery of methionine dependence of cancer; the molecular basis for the increased methionine demand of cancer cells and tumors; the clinical application of methionine dependence of cancer in PET imaging with [11C]methionine; the development of methioninases as cancer drugs; the anti-aging and anti-diabetes effects of methionine restriction; and the future of targeting methionine in the body for the elimination of cancer and for the extension of a healthy life-span. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, MethionineDependence of Cancer and Aging: Methods and Protocols is an important resource for researchers and scientists wishing to pursue this exciting and vital area of study."

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Hoffman, Robert M.

Inhaltsverzeichnis

Methods to Study the Role of Methionine Restricted Diet and Methioninase in Cancer Growth Control.- Altered Methionine Metabolism in Cancer Cells.- Linkage of Methionine-Dependence and Other Features of Malignancy.- Isolation and Characterization of Methionine Independent Clones from Methionine Dependent Cancer Cells.- Tumor-Specific S/G2-Phase Cell Cycle Arrest of Cancer Cells by Methionine Restriction.- Preclinical Breast Cancer Models to Investigate Metabolic Priming by Methionine Restriction.- Efficacy of Methionine-Restricted Diets on Cancers In Vivo.- Dietary Methionine-Restriction Based Cancer Chemotherapy in Rodents.- Clinical Studies of Methionine-Restricted Diets for Cancer Patients.- Development of Recombinant Methioninase for Cancer Treatment.- Methioninase Cell-Cycle Trap Cancer Chemotherapy.- High Efficacy of Recombinant Methioninase on Patient-Derived Orthotopic Xenograft (PDOX) Mouse Models of Cancer.- Total Methionine Restriction Treatment of Cancer.- Methioninase Gene Therapy.- Selenomethionine and Methioninase: Selenium Free Radical Anti-Cancer Activity.- Super and Toxicity of Recombinant Methioninase and Polyethylene Glycol (PEG) Recombinant Methioninase in Primates.- Pilot Phase 1 Clinical Trial of Methioninase on High-Stage Cancer Patients: Rapid Depletion of Circulating Methionine.- Methionine Gamma Lyase from Clostridium sporogenes Increases the Anti-Cancer Effect of Doxorubicin in A549 Cancer Cells In Vitro and Human Cancer Xenografts.- Methionine Restriction and Life-Span Extension.- L-[methyl-11C] Methionine-Positron Emission Tomography (MET PET).- Is the Hoffman Effect for Methionine Overuse Analogous to the Warburg Effect for Glucose Overuse in Cancer?.- Recombinant Methioninase as a DNA Demethylation Agent.- Homocysteine, Thioretinaco Ozonide and Oxidative Phosphorylation in Cancer and Aging: A Proposed Clinical Trial Protocol.- Afterword - Oral Methioninase: Answer to Cancer and Foundation of Youth?.