Liver Proteomics

Methods and Protocols
Softcover Nachdruck of the original 1. Auflage 2012
ISBN: 978-1-4939-6214-3
Verlag: Humana Press

Buch, Englisch, Band 909, 346 Seiten, Previously published in hardcover, Format (B × H): 178 mm x 254 mm, Gewicht: 677 g

Reihe: Methods in Molecular Biology

The liver is responsible for a wide range of critical functions essential to life, and is composed of several different cell types. In  Liver Proteomics: Methods and Protocols, expert researchers in the field detail many of the methods that are used to study the live. These methods include the  most up-to-date strategies being used to characterize the liver proteome at the global, cellular, subcellular, post translational and functional level.Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Liver Proteomics: Methods and Protocols seeks to aid scientists in the further study of this crucially important organ.

Zielgruppe

Professional/practitioner

Autoren/Hrsg.

Hixson, Douglas C.

Josic, Djuro

Fachgebiete

• Naturwissenschaften Biowissenschaften Proteinforschung
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Vorklinische Medizin: Grundlagenfächer Molekulare Medizin, Zellbiologie
• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Klinische und Innere Medizin Hepatologie
• Naturwissenschaften Biowissenschaften Zellbiologie

Inhaltsverzeichnis

Cell Surface Capturing (CSC) technologies for the surfaceome discovery of hepatocytes.-An on-target desalting and concentration sample preparationprotocol for MALDI-MS and MS/MS analysis.-Plasma membrane isolation using immobilized concanavalin A magnetic beads.-Analysis of the cattle liver proteome by High-sensitive Liquid Chromatography Coupled with Mass Spectrometry method.-Producing a One-Dimensional Proteomic Map for Human Liver Cytochromes P450.-Assessing heterogeneity of peroxisomes – isolation of two subpopulations from rat liver.-Purification and Proteomic Analysis of Liver Membrane Skeletons.-Liver Plasma Membranes: An effective method to analyze membrane proteome.-Quantitative analysis of liver Golgi proteome in the cell cycle.-Glycoproteins and Glycosylation: Apolipoprotein C3 Glycoforms by top-down MALDI-TOF Mass Spectrometry.-Phosphoproteomic Analysis of Liver Homogenates.-A combination of affinity chromatography, 2D DIGE and mass spectrometry to analyze the phosphoproteome of liver progenitor cells.-Identifying Acetylated Proteins in Mitosis.-Comparative proteome analysis of a human liver cell line stably transfected with Hepatitis D virus full-length cDNA.-Identification of new autoimmune hepatitis-specific autoantigens by using protein microarray technology.-Proteomics analysis of human non-alcoholic fatty liver.-Proteomic Methods for Biomarker Discovery in a Rat Model of Alcohol Steatosis.-Targeted mass spectrometry-based metabolomic profiling through Multiple Reaction Monitoring of Liver and other biological matrices.-Discovery of Lamin B1 and Vimentin as CirculatingBiomarkers for Early Hepatocellular Carcinoma.-Combinatorial peptide ligand libraries to discover liver disease biomarkers in plasma samples.-Isolation of urinary exosomes from animal models to unravel non-invasive disease biomarker.