PCR Protocols in Molecular Toxicology

1. Auflage 1997
ISBN: 978-0-8493-3344-6
Verlag: CRC Press

Buch, Englisch, 252 Seiten, Format (B × H): 157 mm x 235 mm, Gewicht: 381 g

Reihe: Methods in Life Sciences - Toxicology Section

Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail:

- Quantitative reverse transcriptase PCR and methods to examine gene expression

- Differential display cloning

- Cloning and library screening by PCR

- Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes

- Basic, non-PCR based molecular biology methods

PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.

Autoren/Hrsg.

Heuvel, John P. van de

Vanden Heuvel, John P.

Fachgebiete

• Medizin | Veterinärmedizin Medizin | Public Health | Pharmazie | Zahnmedizin Medizinische Fachgebiete Pharmakologie, Toxikologie
• Naturwissenschaften Biowissenschaften Molekularbiologie

Inhaltsverzeichnis

Chapter 1. The Basics of the Polymerase Chain Reaction -- /William B. Mattes -- Chapter 2. Analysis of Gene Expression -- /John P. Vanden Heuoel -- Chapter 3. Differential Display PCR -- /J. Christopher Corton -- Chapter 4. Cloning by PCR -- /John P. Vanden Heuuel -- Chapter 5. Genotype Analysis -- /Douglas A. Bell and Gary Pittman -- Chapter 6. General Molecular Biology Techniques -- /John P. Vanden Heuvel -- Appendix I — List of Suppliers -- Appendix II — Companies, Online Catalogs, and Internet Resources -- Index.

Über Autor(innen)

Vanden Heuvel, John P.